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Gene Expression Data
Assay Details
Assay
Reference: J:198558 Li W, et al., Tissue-specific venous expression of the EPH family receptor EphB1 in the skin vasculature. Dev Dyn. 2013 Aug;242(8):976-88
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6150924
Gene symbol: Efnb2
Gene name: ephrin B2
Reporter: lacZ
Antibody: anti-B-galactosidase (0855976)
Detection system: Secondary antibody
Results
Specimen 3A: embryonic day 13.5; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: arterial blood vessel Present Regionally restricted 3A Expressed in the arterial endothelial cells. Expressed in branched vessels.
Specimen 3B: embryonic day 14.0; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS22: arterial blood vessel Present Regionally restricted 3B Expressed in the arterial endothelial cells. Expressed in branched vessels. Some positive honeycomb-like vessels appear to be in the process of vascular remodeling and express Ephb1.
Specimen 3C: embryonic day 15.5; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: arterial blood vessel Present Regionally restricted 3C Expressed in the arterial endothelial cells. No significant expression of Ephb1 in the lacZ-positive arterial vasculature.
Specimen 3I: embryonic day 13.5; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925).
Structure Level Pattern Image Note
TS21: arterial blood vessel Present Regionally restricted 3I Expressed in the arterial endothelial cells. Expressed in branched vessels.
Specimen 3J: embryonic day 14.0; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925).
Structure Level Pattern Image Note
TS22: arterial blood vessel Present Regionally restricted 3J Expressed in the arterial endothelial cells. Expressed in branched vessels. Some positive honeycomb-like vessels appear to be in the process of vascular remodeling and express Ephb1.
Specimen 3K: embryonic day 15.5; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925).
Structure Level Pattern Image Note
TS23: arterial blood vessel Present Regionally restricted 3K Expressed in the arterial endothelial cells. No significant expression of Ephb1 in the lacZ-positive arterial vasculature.
Specimen 7B/H: postnatal adult; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: arterial system endothelium Present 7B , 7H , 7H inset
Specimen 9F/H: embryonic day 15.5; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: green - Efnb2; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: arterial blood vessel Present Regionally restricted 9F , 9H Expressed in the arterial endothelial cells.
TS23: lung epithelium Present Regionally restricted 9H , 9F Expressed in distal epithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory