Antibody:	Anti-ONECUT2 (AF6294)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: retina	Present			Expression was detected in the nuclei.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	1G	Expression was detected in both the ganglion cell layer and the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	1H	Expression was detected in both the ganglion cell layer and the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	1I	Expression was detected mainly in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		1J	Expression in the ganglion cell layer was still high at P0.
TS27: retina layer	Present	Regionally restricted	1J	Expression was detected in a row of regularly spaced cells located in the outer part of the retina.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak		1K	Expression in the ganglion cell layer decreased markedly at P5.
TS28: retina layer	Present	Regionally restricted	1K	Expression was detected in a row of regularly spaced cells located in the outer part of the retina.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak		1L	Expression in the ganglion cell layer decreased markedly at P16.
TS28: retina inner nuclear layer	Present	Regionally restricted	1L	Expression was detected in the inner nuclear layer close to the boundary of the inner nuclear layer and outer plexiform layer.

Structure	Level	Pattern	Image	Note
TS20: retina	Weak	Regionally restricted	2A''' , 2A'' , 2A	There was 100% overlap of expression with Oc1, but Oc2 was weaker in most cells of the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	2B''' , 2B'' , 2B	All cells in the ganglion cell layer express both genes. There were more cells expressing Oc1 than Oc2 in the neuroblast layer, but essentially all Oc2-positive cells were also Oc1-positive.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	2C''' , 2C'' , 2C	The overlap of Oc1 and Oc2 expression remained close to 100% in both the ganglion cell layer and the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present			The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer.
TS27: retina layer	Present	Regionally restricted		The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak			The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer, although expression gradually decreased.
TS28: retina layer	Present	Regionally restricted		The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak			The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer, although expression gradually decreased.
TS28: retina layer	Present	Regionally restricted		The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted		There was almost complete overlap of expression with Math5 in the neuroblast layer of the central retina.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4A , 4A''' , 4A''	There was almost complete overlap of expression with Math5 in the neuroblast layer of the central retina. Expression was also detected in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	4B , 4B'' , 4B'''	Expression was detected in the ganglion cells layer. In the neuroblast layer, expression became very low. The majority of Math5-positive cells were negative for Oc2, although a few Oc2-positive/Math5-negative cells could be observed.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4C'' , 4C''' , 4C	Expression completely overlapped with Isl1 in the ganglion cell layer. Only a proportion of Oc2-expressing cells in the neuroblast layer expressed Isl1.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	4D'' , 4D , 4D'''	Co-expression with Isl1 in the ganglion cell layer continued at E14.5. Very little overlap in expression was observed in the neuroblast layer due to the low levels of Oc2.