Antibody:	Anti-HNF-6 (H-100) sc-13050
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	2A' , 2A , 2A'''	There was 100% overlap of expression with Oc1, but Oc2 was weaker in most cells of the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	2B' , 2B , 2B'''	All cells in the ganglion cell layer express both genes. There were more cells expressing Oc1 than Oc2 in the neuroblast layer, but essentially all Oc2-positive cells were also Oc1-positive.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	2C''' , 2C , 2C'	The overlap of Oc1 and Oc2 expression remained close to 100% in both the ganglion cell layer and the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present			The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer.
TS27: retina layer	Present	Regionally restricted		The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak			The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer, although expression gradually decreased.
TS28: retina layer	Present	Regionally restricted		The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak			The overlap of Oc1 and Oc2 expression remained close to 100% in the ganglion cell layer, although expression gradually decreased.
TS28: retina layer	Present	Regionally restricted		The overlap of Oc1 and Oc2 expression remained close to 100% in the presumed horizontal cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	5B , 5C	Expression was detected in horizontal cells. There was complete overlap with the expression of the horizontal cell marker Calbindin.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	5E , 5F	Expression was detected in horizontal cells. There was complete overlap with the expression of the horizontal cell marker Lim1.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	5M , 5M''' , 5M'	Expression was detected in Lim1-positive horizontal cell precursors.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	6A , 6A''' , 6A'	Expression was detected in the newly forming ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	6C' , 6C''' , 6C	Expression was detected in some M-phase cells.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	6D''' , 6D' , 6D	At E14.5, many more expressing cells became postmitotic.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	6F' , 6F , 6F'''	At E14.5, many more expressing cells became postmitotic.

Structure	Level	Pattern	Image	Note
TS22: retina	Strong	Regionally restricted	8A	Expression was detected in a narrow band in the inner most regions of the retina.

Structure	Level	Pattern	Image	Note
TS22: retina	Present		8B

Structure	Level	Pattern	Image	Note
TS22: retina	Present		8C

Structure	Level	Pattern	Image	Note
TS22: retina	Strong	Regionally restricted	8D	Expression was detected in a narrow band in the inner most regions of the retina as in wild type. There was no change in the level or pattern of expression in the mutant.

Structure	Level	Pattern	Image	Note
TS22: retina	Present		8E	There was no change in the expression pattern or level in the mutant compared to the control.

Structure	Level	Pattern	Image	Note
TS22: retina	Present		8F	There was no change in the expression pattern or level in the mutant compared to the control.