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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450737
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Probe: MGC clone 90732
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3c: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-1; brown - Arx (assay MGI:4452072).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted 3c , 3d Expression is present in the hypothalamic zone running parallel to the basal plate and in posterior hypothalamus.
Specimen 3f: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-1; brown - Lhx9 (assay MGI:4452073).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted 3f , 3g Expression is present in the hypothalamic zone running parallel to the basal plate and in posterior hypothalamus.
Specimen 3j: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-1; brown - Lhx6 (assay MGI:4452075).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted 3j Expression is present in the intrahypothalamic diagonal.
TS20: diencephalon Present Regionally restricted 3j Expression is present in the tuberomammillary terminal.
Specimen S6k: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted S6_k Expression is present in the basal hypothalamus.
Specimen S6l: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: blue - Nkx2-1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Absent S6_l There is no expression in the basal hypothalamus.
Specimen Nkx 2.1 - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Ambiguous NKX2.1(D)+SHH(F)-E11.5-CORONAL , NKX2.1(D)+SHH(F)-E11.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E11.5-CORONAL , NKX2.1(D)+SHH(F)-E11.5-SAGITTAL There is strong staining in the VZ of the dorsal & ventral POAs, VMH & ARH nuclei, AVH, anterior & posterior IDs, premam & mam zones & TT. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, PVH nucleus & supramam zone.
TS19: telencephalon mantle layer Ambiguous NKX2.1(D)+SHH(F)-E11.5-CORONAL , NKX2.1(D)+SHH(F)-E11.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E11.5-SAGITTAL , NKX2.1(D)+SHH(F)-E11.5-CORONAL There is strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
Specimen Nkx 2.1 - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous NKX2.1(D)+SHH(F)-E12.5-CORONAL , NKX2.1(D)+SHH(F)-E12.5-Sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous NKX2.1(D)+SHH(F)-E12.5-CORONAL , NKX2.1(D)+SHH(F)-E12.5-Sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous NKX2.1(D)+SHH(F)-E12.5-Sagittal , NKX2.1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous NKX2.1(D)+SHH(F)-E12.5-CORONAL , NKX2.1(D)+SHH(F)-E12.5-Sagittal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E12.5-CORONAL , NKX2.1(D)+SHH(F)-E12.5-Sagittal There is strong staining in the ventricular zone of the premam and mam zones and the TT. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS20: ganglionic eminence Strong Non-Uniform NKX2.1(D)+SHH(F)-E12.5-Sagittal , NKX2.1(D)+SHH(F)-E12.5-CORONAL There is strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous NKX2.1(D)+SHH(F)-E12.5-Sagittal , NKX2.1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E12.5-CORONAL , NKX2.1(D)+SHH(F)-E12.5-Sagittal There is strong staining in the ventricular zone of the dorsal and ventral POAs, the VMH and ARH nuclei, the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the PVH nucleus.
TS20: thalamus mantle layer Ambiguous NKX2.1(D)+SHH(F)-E12.5-Sagittal , NKX2.1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Ambiguous NKX2.1(D)+SHH(F)-E12.5-Sagittal , NKX2.1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Nkx 2.1 - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL There is strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
TS22: diencephalon ventricular layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL There is strong staining in the ventricular zone of the premam and mam zones and the TT. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS22: ganglionic eminence Strong Non-Uniform NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL There is strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Strong Non-Uniform NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL There is strong staining in the ventricular zone of the dorsal and ventral POAs, the VMH and ARH nuclei, the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the PVH nucleus.
TS22: thalamus mantle layer Ambiguous NKX2.1(D)+SHH(F)-E14.5-SAGITTAL , NKX2.1(D)+SHH(F)-E14.5-CORONAl Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous NKX2.1(D)+SHH(F)-E14.5-CORONAl , NKX2.1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Nkx 2.1 - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous NKX2.1(D)+SHH(F)-E16.5-CORONAl , NKX2.1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous NKX2.1(D)+SHH(F)-E16.5-CORONAl , NKX2.1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous NKX2.1(D)+SHH(F)-E16.5-CORONAl , NKX2.1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Very strong Non-Uniform NKX2.1(D)+SHH(F)-E16.5-SAGITTAL , NKX2.1(D)+SHH(F)-E16.5-CORONAl There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zone.
TS24: diencephalon ventricular layer Very strong Non-Uniform NKX2.1(D)+SHH(F)-E16.5-SAGITTAL , NKX2.1(D)+SHH(F)-E16.5-CORONAl There is very strong staining in the ventricular zone of the mam zone and moderate, scattered staining in the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS24: ganglionic eminence Very strong Non-Uniform NKX2.1(D)+SHH(F)-E16.5-SAGITTAL , NKX2.1(D)+SHH(F)-E16.5-CORONAl There is very strong staining in the ventricular zone of the ganglionic eminences and moderate, scattered staining in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer Moderate Non-Uniform NKX2.1(D)+SHH(F)-E16.5-SAGITTAL , NKX2.1(D)+SHH(F)-E16.5-CORONAl There is moderate staining in the MZ of the AVH and moderate, scattered staining in the dorsal POA and ARH nucleus. Staining is either absent or ubiquitous in the MZ of the ventral POA, the PVH, SCH, DMH and VMH nuclei, the DAH and the PH.
TS24: hypothalamus ventricular layer Very strong Non-Uniform NKX2.1(D)+SHH(F)-E16.5-SAGITTAL , NKX2.1(D)+SHH(F)-E16.5-CORONAl There is very strong staining in the VZ of the dorsal and ventral POAs and moderate, scattered staining in the VMH and ARH nuclei. Staining is either absent or ubiquitous in the VZ of the PVH, SCH and DMH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Ambiguous NKX2.1(D)+SHH(F)-E16.5-CORONAl , NKX2.1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous NKX2.1(D)+SHH(F)-E16.5-SAGITTAL , NKX2.1(D)+SHH(F)-E16.5-CORONAl Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
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