Gene Expression Data
Assay Details
Assay
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Reference:
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J:79514
Burke Z, et al., Prox1 is an early specific marker for the developing liver and pancreas in the mammalian foregut endoderm. Mech Dev. 2002 Oct;118(1-2):147
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3529593
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Gene symbol:
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Prox1
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Gene name:
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prospero homeobox 1
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase (55976)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4A 1A:
embryonic day 8.5; Prox1tm1Gco/Prox1 +
(more )
Note: This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Foxa2 (assay MGI:3529577). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
Specimen
4A 1A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * NMRI
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Foxa2 (assay MGI:3529577). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
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Structure |
Level |
Pattern |
Image |
Note |
TS13: hindgut diverticulum |
Present |
Regionally restricted |
4A 1A
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Expression is present in the endoderm of the dorsal hindgut.
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Specimen
4A 2A:
embryonic day 8.5; Prox1tm1Gco/Prox1 +
(more )
Note: This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Foxa2 (assay MGI:3529577). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
Specimen
4A 2A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * NMRI
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Foxa2 (assay MGI:3529577). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
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Specimen
4A 3A:
embryonic day 8.5; Prox1tm1Gco/Prox1 +
(more )
Note: This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Foxa2 (assay MGI:3529577). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
Specimen
4A 3A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * NMRI
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Foxa2 (assay MGI:3529577). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
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Specimen
4B 1B:
embryonic day 8.5; Prox1tm1Gco/Prox1 +
(more )
Note: This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Shh (assay MGI:3529582). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
Specimen
4B 1B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * NMRI
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
|
This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Shh (assay MGI:3529582). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
|
Specimen
4B 2B:
embryonic day 8.5; Prox1tm1Gco/Prox1 +
(more )
Note: This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Shh (assay MGI:3529582). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
Specimen
4B 2B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * NMRI
|
Mutant Allele(s):
|
Prox1tm1Gco/Prox1+
|
Age:
|
embryonic day 8.5
|
Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
|
Note:
|
This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Shh (assay MGI:3529582). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
|
Specimen
4B 3B:
embryonic day 8.5; Prox1tm1Gco/Prox1 +
(more )
Note: This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Shh (assay MGI:3529582). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
Specimen
4B 3B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * NMRI
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
|
embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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This embryo contains 9-10 somites. Double labeled: brown - Prox1; blue - Shh (assay MGI:3529582). After whole mount staining, this embryo was embedded in gelatin and sucrose for cryosectioning.
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Structure |
Level |
Pattern |
Image |
Note |
TS13: notochord |
Present |
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4B 3B
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Expression is present in the posterior notochord.
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