Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:73288
Sonnenberg-Riethmacher E, et al., Development and degeneration of dorsal root ganglia in the absence of the HMG-domain transcription factor Sox10. Mech Dev. 2001 Dec;109(2):253-65
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:3039657
|
|
Gene symbol:
|
Sox10
|
|
Gene name:
|
SRY (sex determining region Y)-box 10
|
|
Reporter:
|
lacZ
|
|
Antibody:
|
anti-B-galactosidase
|
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
Assay notes:
|
Embryos were fixed in 1% paraformaldehyde.
|
|
|
Results
|
Specimen
5A:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more  )
Note: Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
Specimen
5A:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
5B:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
5C:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: red - Sox10 reporter; green - Prph (assay MGI:3039466). Embryos were fixed in 1% paraformaldehyde.
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Prph (assay MGI:3039466). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
5D:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: red - Sox10 reporter; green - Prph (assay MGI:3039466). Embryos were fixed in 1% paraformaldehyde.
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Prph (assay MGI:3039466). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
5E:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: red - Sox10 reporter; green - Isl1 (assay MGI:3039467). Embryos were fixed in 1% paraformaldehyde.
Specimen
5E:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Isl1 (assay MGI:3039467). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
5F:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: red - Sox10 reporter; green - Isl1 (assay MGI:3039467). Embryos were fixed in 1% paraformaldehyde.
Specimen
5F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Isl1 (assay MGI:3039467). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
6C:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
Specimen
6C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
6D:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
Specimen
6D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Nefm (assay MGI:3039463). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
7A:
embryonic day 8.75; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: red - Sox10 reporter; green - Ngfr (assay MGI:3039468).
Specimen
7A:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 8.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Ngfr (assay MGI:3039468).
|
Specimen
7B:
embryonic day 10.0; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: red - Sox10 reporter; green - Ngfr (assay MGI:3039468).
Specimen
7B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Ngfr (assay MGI:3039468).
|
Specimen
7C:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: red - Sox10 reporter; green - Ngfr (assay MGI:3039468).
Specimen
7C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Sox10 reporter; green - Ngfr (assay MGI:3039468).
|
Specimen
8B:
embryonic day 10.0; Sox10tm1Weg/Sox10 +
(more )
Specimen
8B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
8C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
8G:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
9E:
embryonic day 11.0; Sox10tm1Weg/Sox10 +
(more )
Specimen
9E:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
9G:
embryonic day 11.0; Sox10tm1Weg/Sox10 +
(more )
Specimen
9G:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools