Caution: These are Prototype Reports for Evaluation Purposes Only!

MGD Full QTL Report

J# 26734 Sundvall et al., 1995

Phenotype Class: Resistance/Susceptibility
Phenotype Mode: Induced
- Induced by: immunization with myelin basic protein (MBP) peptide 89-101
Phenotype Name: Chronic Experimental Autoimmune Encephalomyelitis
- Phenotype Trait 1: Susceptibility to EAE
- Phenotype Assessment: semi-quantitative
Alternative Keywords: EAE, MS, multiple sclerosis
Strain/Progenitor Information:
- Strain 1: B10.RIII
- Strain 1 Comment: susceptible to chronic, relapsing EAE
- Strain 2:RIIIS/J
- Strain 2 Comment: resistant to chronic, relapsing EAE
Phenotype Comments: NULL

Progeny Generation Used for QTL Experiment: F2
Progeny Population Symbol: NULL
# Animals Evaluated: 92 + 92
Female Parent: (B10.RII X RIIIS/J)F1
Male Parent: (B10.RII X RIIIS/J)F1
Cross type: intercross
Another Mapping Cross for this Progeny: No
Comments:
- 1) First pass included 92 most severly affected animals in screen, subsequently 92 additional F2 intercross animals were typed.
- 2) because both strains carry the H-2^r haplotype, QTL eae1 does not segregate in the cross

Chromosomes Included in Marker Map Coverage: 1,6,7,9,11,14,17
Marker Map Methods:
- PCR-microsatellites
Number of Markers in Analysis: 168
Intermarker Distance: mean distance between 6.5 and 20.4 cM
Comments on Marker Map: NULL
QTL Detection Method:
- Chi-square Contingency tests
- LOD
Significance Threshold for QTL Detection:NULL
- Significance Threshold Comment: NULL
QTL Estimation Method: NULL
Comments on QTL Estimation Model Used: NULL
QTL Mapping Software:
- MapMaker/EXP

Table of Linked Markers:

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
eae2	D15Mit11	11.46	=0.0032	15
eae2	D15Mit51	13.23	=0.0013	15
eae2	D15Mit53	13.96	=0.0009	15
eae2	D15Mit52	13.18	=0.0013	15
eae2	D15Nds2	16.94	=0.00021	15
eae2	D15mit82	11.04	=0.0040	15
eae2	D15Mit111	9.20	=0.0100	15
eae2	D15Mit7	7.14	=0.0281	15
eae2	D15Mit8	8.28	=0.0159	15
eae2	D15Mit56	10.14	=0.0062	15

QTL Estimation
- Chromosome: 15
- Location:Peak LOD score of 3.89 near D15Nds2
- % Phenotypic Variation Accounted For: NULL
- Confidence Interval for QTL Estimation: NULL
QTL Estimation: Chromosome 3
- Chromosome: 3
- Location: NULL
- % Phenotypic Variation Accounted For:NULL
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Results consistent with the prescence of a dominant gene with reduced penetrance in B10.RIII at the eae2 locus and that other genes are important for disease susceptibility.
- 2) eae2 region syntenic to human chromosome 5p14-12
- 3) eae2 has strongest effect with present in heterozygous state
- 4) P values not corrected for multiple tests

Table of Linked Markers:

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
eae3	D3Mit76	7.13	=0.0283	3
eae3	D3Mit75	9.68	=0.0079	3
eae3	D3Mit101	12.09	=0.0024	3
eae3	D3Mit103	11.18	=0.0037	3
eae3	D3Mit105	9.28	=0.0096	3

QTL Estimation
- Chromosome: 3
- Location: Peak LOD score of 2.85 near D3Mit101
- % Phenotypic Variation Accounted For: NULL
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Results suggest the an additive mode of inheritance for the eae3 locus
- 2) P values not corrected for multiple tests

QTL Symbol(s)	Candidate Gene Symbol	Chromosome	Gene Name
eae2	Il7r	15	interleukin-7 receptor
eae2	Ghr	15	growth hormone receptor
eae2	Prlr	15	prolactin receptor
eae2	Lifr	15	leukemia inhibitory receptor
eae2	C6	15	complement factor 6
eae2	C7	15	complement factor 7
eae3	Fcgr1	3	high affinity Fc receptor for IgG
eae3	Il6ra	3	interleukin 6 receptor
eae3	Csfm	3	macrophage colony stimulating factor

1) Results suggest that eae2 and eae3 loci interact to cause disease
2) Many of the genes in the candidate regions on chromosomes 3 and 15 are known to be involved in B cell differentiation