Caution: These are Prototype Reports for Internal Evaluation Purposes Only!

MGD Full QTL Report

J# 24238 Warden et al., 1995

Bibliographic information for J24238

Phenotype Information

Progeny Population Information

Methods

QTL Data

Candidate Genes

General Comments

Phenotype Information for J24238

Phenotype Class: Disease
Phenotype Mode: Hereditary
Phenotype Name: Multifactorial obesity
- Phenotype Trait 1: Body weight (gm)
- Phenotype Assessment: quantitative
- Phenotype Trait 2: Anal-nasal length (cm)
- Phenotype Assessment: quantitative
- Phenotype Trait 3: Body mass index
- Phenotype Assessment: quantitative
- Phenotype Trait 4: Parametrial fat pad (gm)
- Phenotype Assessment: quantitative
- Phenotype Trait 5: Retroperitoneal fat pad (gm)
- Phenotype Assessment: quantitative
- Phenotype Trait 6: Mesenteric fat pad (gm)
- Phenotype Assessment: quantitative
- Phenotype Trait 7: Femoral fat pad (gm)
- Phenotype Assessment: quantitative
- Phenotype Trait 8: Percent body fat
- Phenotype Assessment: quantitative
- Phenotype Trait 9:Total cholesterol (mg/dl)
- Phenotype Assessment: quantitative
- Phenotype Trait 10:LDL cholesterol (mg/dl)
- Phenotype Assessment: quantitative
- Phenotype Trait 11: Hepatic lipase activity (uU/ml)
- Phenotype Assessment: quantitative
- Phenotype Trait 12: Plasma insulin
- Phenotype Assessment: quantitative
- Phenotype Trait 13: Plasma glucose
- Phenotype Assessment: quantitative
- Phenotype Trait 14: Plasma corticosterone
- Phenotype Assessment: quantitative
- Phenotype Trait 15: Percent body water
- Phenotype Assessment: quantitative
- Phenotype Trait 16: Percent body protein
- Phenotype Assessment: quantitative
Alternative Keywords: obesity, multigenic obesity
Strain/Progenitor Information:
- Strain 1:C57BL/6J
- Strain 1 Comment: NULL
- Strain 2: SPRET/Pt
- Strain 2 Comment: NULL
- Strain 1: B10.129(5M)/nSn
- Strain 1 Comment: congenic on genetic background of C57BL/10SnJ
- Strain 1: B10.C(41N)/Sn
- Strain 1 Comment: congenic on genetic background of C57BL/10SnJ
Phenotype Comments: NULL

Progeny Population for J24238

Population 1

Progeny Generation Used for QTL Experiment: Backcross (N1)
Progeny Population Name: SPRET/Pt BSB
# Animals Evaluated: 215
Female Parent: (C57BL/6 X SPRET/Pt)F1
Male Parent: C57BL/6J
Cross Type: Backcross
Comments:
- 1) plasma hepatic lipase activity measured only in SPRET/Pt BSB mice
- 2) SPRET/Pt BSB mice sacrificed using ether; SPRET/Ei BSB mice sacrified using isoflurane

Another Mapping Cross for this Progeny?: Yes

Population 2

Progeny Generation Used for QTL Experiment: Backcross (N1)
Progeny Population Name: SPRET/Ei BSB
# Animals Evaluated: 197
Female Parent: (C57BL/6 X SPRET/Ei)F1
Male Parent: C57BL/6J
Cross Type: Backcross
Comments:
- 1) plasma hepatic lipase activity measured only in SPRET/Pt BSB mice
- 2) SPRET/Pt BSB mice sacrificed using ether; SPRET/Ei BSB mice sacrified using isoflurane

Another Mapping Cross for this Progeny?: No

Methods for J24238

Chromosomes Included in Marker Map Coverage: All but the Y
Marker Map Methods:
- PCR- SSLV
Number of Markers in Analysis: 148
Intermarker Distance: Approximately 10 cM
Comments on Marker Map:
- 1) The two substrains of Mus spretus have identical allele sizes for > 99% of SSLVs
- 2) Genes associated with obesity should be within 5 cM of a typed marker
QTL Detection Method: NULL
Significance Threshold for QTL Detection: NULL
- Significance Threshold Comment: NULL
QTL Estimation Method: interval mapping
Comments on QTL Estimation Model Used: NULL
QTL Mapping Software: MapMaker
Comments: NULL

QTL Information for J24238

Phenotype Trait: Percent body fat

QTL Symbol: Mob-1

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-1 D7Mit8 4.2 NULL 7
QTL Estimation
- Chromosome: 7
- Location: Peak LOD of 4.2 at D7Mit8
- % Phenotypic Variation Accounted For: 6.5%
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Heterozygotes for SPRET and B6 alleles at D7Mit8 had 25% more body fat than mice homozygous for B6 alleles.

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-1	D7Mit8	4.2	NULL	7

QTL Information for J24238

Phenotype Trait: Hepatic lipase acitivity

QTL Symbol: Mob-1

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-1 D7Mit8 5.1 NULL 7
QTL Estimation
- Chromosome: 7
- Location: Peak LOD of 5.1 at distal half of chromosome 7
- % Phenotypic Variation Accounted For: 9%
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) This linkage is idependent of the hepatic lipase structural gene on chromosome 9.
- 2) Heterozygotes at D7Mit8 had 35% higher hepatic lipase activity than mice homozygouse for B6 alleles.

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-1	D7Mit8	5.1	NULL	7

QTL Information for J24238

Phenotype Trait: Total plasma cholesterol

QTL Symbol: Mob-1

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-1 D7Mit8 4.4 NULL 7
QTL Estimation
- Chromosome: 7
- Location: Peak LOD score of 4.4 at D7Mit8
- % Phenotypic Variation Accounted For: NULL
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Heterozygotes for SPRET and B6 alleles at D7Mit8 had 25% more body fat than mice homozygous for B6 alleles.

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-1	D7Mit8	4.4	NULL	7

QTL Information for J24238

Phenotype Trait: Percent body water

QTL Symbol: Mob-1

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-1 D7Mit8 4.6 NULL 7
QTL Estimation
- Chromosome: 7
- Location: Peak LOD score of 4.6 at D7Mit8
- % Phenotypic Variation Accounted For: NULL
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Heterozygotes for SPRET and B6 alleles at D7Mit8 had 25% more body fat than mice homozygous for B6 alleles.

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-1	D7Mit8	4.6	NULL	7

QTL Information for J24238

Phenotype Trait: Femoral fat pad weight

QTL Symbol: Mob-2

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-2 D6Mit1 4.8 NULL 6
QTL Estimation
- Chromosome: 6
- Location: Peak LOD score of 4.8 at D6Mit1
- % Phenotypic Variation Accounted For: 7.1%
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Heterozygotes at D6Mit1 had femoral fat pads that were about 2X heavier than mice homozygous for B6 alleles.

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-2	D6Mit1	4.8	NULL	6

QTL Information for J24238

Phenotype Trait: Total plasma cholesterol

QTL Symbol: Mob-2

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-2 D6Mit1 3.4 NULL 6
QTL Estimation
- Chromosome: 6
- Location: Peak LOD score of 3.4 at D6Mit1
- % Phenotypic Variation Accounted For: NULL
- Confidence Interval for QTL Estimation: NULL
Comments: NULL

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-2	D6Mit1	3.4	NULL	6

QTL Information for J24238

Phenotype Trait: Percent body fat

QTL Symbol: Mob-3

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-3 D12Mit27 4.8 NULL 12
QTL Estimation
- Chromosome: 12
- Location: Peak LOD score of 4.8 at D12Mit27
- % Phenotypic Variation Accounted For: 7%
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Heterozygotes at D12Mit27 had 44% greater body fat than mice homozygous for B6 alleles.
- 2) SPRET/Ei mice were neutral for linkage of Mob-3 and D12Mit27

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-3	D12Mit27	4.8	NULL	12

QTL Information for J24238

Phenotype Trait: Mesenteric fat pads weight

QTL Symbol: Mob-4

QTL Detection Method: LOD
Table of Linked Markers:

QTL Symbol Linked Marker Symbol Association Score P Value Chromosome

Mob-4 D15Mit13 3.4 NULL 15
QTL Estimation
- Chromosome: 15
- Location: Peak LOD score of 3.4 at D15Mit13
- % Phenotypic Variation Accounted For: 5.9%
- Confidence Interval for QTL Estimation: NULL
Comments:
- 1) Heterozygotes had 32% smaller mesenteric fat pads than mice homozygous for B6 alleles.

QTL Symbol	Linked Marker Symbol	Association Score	P Value	Chromosome
Mob-4	D15Mit13	3.4	NULL	15

Candidate Genes for QTLs Reported in J24238

QTL Symbol(s)	Candidate Gene Symbol	Chromosome	Gene Name
Mob1	Snrpn	7	small nuclear ribonucleoprotein N
Mob1	Iglfr	7	insulin-like growth factor I receptor
Mob1	tub	7	tubby
Mob1	Ath3	7	atherosclerosis 3
Mob2	(ob) Lep	6	leptin
Mob3	Tshr	12	thyroid stimulating hormone, receptor
Mob4	Ghr	15	growth hormone receptor

Comments:NULL

Overall Comments for J24238

1) Mob-1 also associated with body mass index, body weight, and HDL cholesterol, but the LOD scores were not statistically significant.
2) Mob-2 shows suggestive linkage with percent body water and retroperitoneal fat, HDL cholesterol and plasma insulin.
3) Mob-3 shows suggestive linkage with percent body water and body weight.
4) Mob-4 shows suggestive linkage with percent body fat.
5) Congenic strains used to identify putative candidate loci for Mob-1

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