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Using the Forward to MouseBLAST Form

The help document answers the following questions about the Forward to MouseBLAST form:

What's the purpose of this form?

Use this query form to search each sequence forwarded to MouseBLAST against a particular sequence database.

MouseBLAST searches a forwarded sequence against a mouse, rat, rodent, or human sequence database to find matching sequences. The output of each search (sequences similar to the query sequence) appears in a separate web browser window. If a matching sequence is associated to an MGI mouse gene record, a summary of that gene and a link to MGI appear in the search results. You can either download sequences for any matches in FASTA format or forward them to MouseBLAST for additional searching.

How do I use it?

For each sequence you want to search, do the following. Use the fields in the last column to specify MouseBLAST search options.
  1. Select whether you wish to mask the query sequence. By default, the sequence is masked. (See Masking Details.)
  2. Select the BLAST program you wish to use. The default program is picked based on whether the query sequence is nucleotide or protein. (See WU-BLAST Programs.)
  3. Select the sequence database you wish to search. (See Sequence Databases.)
  4. Click Search. Results of the search will appear in a new browser window.
Alternatively, you may also search the sequence using the MouseBLAST Query Form by following the Advanced BLAST query form link.

How do I interpret the results?

The results of each search appear in a new web browser window. See Using The MouseBLAST Output Report for details.

Are there any sample searches?

See Example MouseBLAST Searches.

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Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/06/2008
MGI_4.11
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