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Reference: J:46439 Freeman TC, MGI Direct Data Submission 1998;():
Assay type: RT-PCR
MGI Accession ID: MGI:1205940
Gene symbol: Kcnma1
Gene name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Modification date: 06/01/1998

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Probe: st97_733
Visualized with: Ethidium bromide
Assay notes: MgCl2: 3.5 mM; Annealing temp: 55C; Annealing time: 90sec; Cycles: x35

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Gel Data
Image: Figure st97_733

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	160 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
Lane 1	P week 6-8	TS28: brain	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 2	P week 6-8	TS28: cerebral cortex	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 3	P week 6-8	TS28: hippocampus	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 4	P week 6-8	TS28: midbrain	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 5	P week 6-8	TS28: corpus striatum	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 6	P week 6-8	TS28: olfactory bulb	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 7	P week 6-8	TS28: cerebellum	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 8	P week 6-8	TS28: spinal cord	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 9	P week 6-8	TS28: pituitary gland	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 10	P week 6-8	TS28: eye	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 11	P week 6-8	TS28: skeletal muscle	Trace	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 12	P week 6-8	TS28: heart	Absent	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 13	P week 6-8	TS28: lung	Absent	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 14	P week 6-8	TS28: spleen	Absent	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 15	P week 6-8	TS28: liver	Absent	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 16	P week 6-8	TS28: gallbladder	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 17	P week 6-8	TS28: pancreas	Absent	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 18	P week 6-8	TS28: kidney	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 19	P week 6-8	TS28: urinary bladder	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 20	P week 6-8	TS28: adrenal gland	Strong	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 21	P week 6-8	TS28: skin	Trace	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 22	P week 6-8	TS28: adipose tissue	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 23	P week 6-8	TS28: femur	Trace	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 24	P week 6-8	TS28: hand	Trace	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 25	P week 6-8	TS28: incisor	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 26	P week 6-8	TS28: tongue	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 27	P week 6-8	TS28: salivary gland	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 28	P week 6-8	TS28: esophagus	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 29	P week 6-8	TS28: stomach fundus	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 30	P week 6-8	TS28: pyloric antrum	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 31	P week 6-8	TS28: duodenum	Trace	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 32	P week 6-8	TS28: jejunum	Trace	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 33	P week 6-8	TS28: ileum	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 34	P week 6-8	TS28: caecum	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 35	P week 6-8	TS28: ascending colon	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 36	P week 6-8	TS28: descending colon	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 37	P week 6-8	TS28: apex of caecum	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 38	P week 6-8	TS28: thymus	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 39 (a)	P week 6-8	TS28: mammary gland	Moderate	0.01 µg; total RNA	C57BL/6J		Female
Lane 40 (b)	P week 6-8	TS28: mammary gland	Moderate	0.01 µg; total RNA	C57BL/6J		Female
Lane 41	P week 6-8	TS28: testis	Moderate	0.01 µg; total RNA	C57BL/6J		Male
Lane 42	P week 6-8	TS28: epididymis	Moderate	0.01 µg; total RNA	C57BL/6J		Male
Lane 43	P week 6-8	TS28: ovary	Strong	0.01 µg; total RNA	C57BL/6J		Female
Lane 44	P week 6-8	TS28: uterine cervix	Absent	0.01 µg; total RNA	C57BL/6J		Female
Lane 45	P week 6-8	TS28: placenta	Moderate	0.01 µg; total RNA	C57BL/6J		Female
Lane 46	E 15-16	TS23: embryo	Moderate	0.01 µg; total RNA	C57BL/6J		Pooled
Lane 47	Control: no data stored
Lane 48	Control: no data stored

Notes:
(a) Non-pregnant
(b) Pregnant