References
Query Results -- Details
MGI Accession ID: MGI:49546
J Number: J:1014
Other Accession IDs:
Title: Construction of a mouse yeast artificial chromosome library in a recombination-deficient strain of yeast.
Authors: Chartier FL; Keer JT; Sutcliffe MJ; Henriques DA; Mileham P; Brown SD
Journal: Nat Genet
Volume: 1
Issue: 2
Date: 1992 May
Year: 1992
Pages: 132-6
Review Status: Peer Reviewed
Abstract:
We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones.
