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Tg(APP695)3Dbo
Transgene Detail

Nomenclature
Symbol: Tg(APP695)3Dbo
Name: transgene insertion 3, David R Borchelt
MGI ID: MGI:2447334
Synonyms: APP695, APP695swe, APPswe, line C3-3, Mo/HuAPPswe
Transgene: Tg(APP695)3Dbo   Location: unknown    
Transgene
origin
Strain of Origin: (C57BL/6J x C3H/HeJ)F2
Transgene
description
Transgene Type: Transgenic (random, expressed)
Mutation: Insertion
  The transgene is composed of a cDNA encoding a chimeric APP protein regulated by the mouse prion promoter. The chimeric APP molecule was created by replacing sequences encoding the Abeta domain of a 695 amino acid isoform of the murine sequence with the cognate sequences of the human gene (mutations K595N, M596L). The human mutations are found in familial Alzheimer's disease. Transgene expression was observed in the brain and heart by Western blot analysis using a monoclonal antibody recognizing the human Abeta region. (J:80782)
Find Mice (IMSR) Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation: Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

      Key:  
hm homozygous ht heterozygous
cn conditional genotype  cx complex: > 1 genome feature
tg involves transgenes ot other: hemizygous, indeterminate,...
N normal phenotype expected model not found
Affected SystemsGenotypes:
 
tg1
 
tg2
 
tg3
 
tg4
 
tg5
 
tg6
 
tg7
 
tg8
 
tg9
 
tg10
 
tg11
 
tg12
  
behavior/neurological          		√ √ √√√     
  
hearing/vestibular/ear          		√           
  
hematopoietic system          		           √
  
immune system          		           √
  
nervous system          		√√√√ √√√√√N√
  
other phenotype          		√√√√N√√√√√ √
  
touch/vibrissae          		  √         
  
vision/eye          		           √
 
  
Disease Models          		√√ √  √√√   
Phenotypic
data by
genotype
Phenotypic Data by Genotype

(show or hide all phenotypic details)

GenotypeAllelic CompositionGenetic Background
  
 tg1   Disease Model  		 Tg(APP695)3Dbo/0 B6.C3-Tg(APP695)3Dbo
  
 tg2   Disease Model  		 Tg(APP695)3Dbo/0 involves: C3H/HeJ * C57BL/6J
  
 tg3   		 Tg(APP695)3Dbo/0
Tg(PSEN1)5Dbo/0		 B6.Cg-Tg(APP695)3Dbo Tg(PSEN1)5Dbo
  
 tg4   Disease Model  		 Klc1tm1Gsn/Klc1+
Tg(APP695)3Dbo/?		 involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6
  
 tg5   		 Bace1tm1Pcw/Bace1tm1Pcw
Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		 involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6J
  
 tg6   		 Bace1tm1Pcw/Bace1+
Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		 involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6J
  
 tg7   Disease Model  		 Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		 involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6J
  
 tg8   Disease Model  		 Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		 involves: C3H/HeJ * C57BL/6J
  
 tg9   Disease Model  		 Tg(APP695)3Dbo/0
Tg(PSEN1)5Dbo/0		 involves: C3H/HeJ * C57BL/6J
  
 tg10   		 Tg(APP695)3Dbo/0
Tg(Eno2-PTGS2)32Pasi/0
Tg(PSEN1)5Dbo/0		 involves: C3H/HeJ * C57BL/6J
  
 tg11   		 Tg(APP695)3Dbo/0
Tg(PSEN1)1Dbo/0		 involves: C57BL/6J * C3H/HeJ
  
 tg12   		 Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		 Not Specified
Disease
models
Mouse Models
of Human Disease
NoteGenotypeRef(s)
 
Allelic Composition
Genetic Background
Models involving transgenes or other mutation types.1
Alzheimer Disease 3
OMIM ID: 607822		  
 
tg8		Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		involves: C3H/HeJ * C57BL/6JJ:123534
 
 
tg9		Tg(APP695)3Dbo/0
Tg(PSEN1)5Dbo/0		involves: C3H/HeJ * C57BL/6JJ:43788
 
 
tg7		Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6JJ:123534
Alzheimer Disease 4
OMIM ID: 606889		  
 
tg8		Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		involves: C3H/HeJ * C57BL/6JJ:123534
 
 
tg9		Tg(APP695)3Dbo/0
Tg(PSEN1)5Dbo/0		involves: C3H/HeJ * C57BL/6JJ:43788
 
 
tg1		Tg(APP695)3Dbo/0B6.C3-Tg(APP695)3DboJ:109847
Alzheimer Disease; AD
OMIM ID: 104300		  
 
tg4		Klc1tm1Gsn/Klc1+
Tg(APP695)3Dbo/?		involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6J:96346
 
 
tg8		Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		involves: C3H/HeJ * C57BL/6JJ:123534
 
 
tg9		Tg(APP695)3Dbo/0
Tg(PSEN1)5Dbo/0		involves: C3H/HeJ * C57BL/6JJ:43788
 
 
tg7		Tg(APP695)3Dbo/0
Tg(PSEN1dE9)S9Dbo/0		involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ * C57BL/6JJ:123534
 
 
tg1		Tg(APP695)3Dbo/0B6.C3-Tg(APP695)3DboJ:109847
 
 
tg2		Tg(APP695)3Dbo/0involves: C3H/HeJ * C57BL/6JJ:87691
1Models involving transgenes or other mutation types may also appear in other sections of the table.
Notes Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 (Tg(Prnp-App/APPswe)E1-2Dbo) and C3-3.

This line was generated from founder number C3-3.

Transgenic mice develop amyloid deposits in brain tissue by 18-20 months of age.

Transgenic mice that are also transgenic for Tg(PSEN1)5Dbo express both human presenilin 1 (A246E variant) and a chimeric amyloid precursor protein (APPSwe) under direction of the mouse prion protein promoter. Elevated levels of the AB1-42(43) peptide are detected in brain homogenates. By nine months of age, histological examination of brain tissue from these mice reveals numerous amyloid deposits resembling those observed in the brains of patients with Alzheimer's disease (AD). The number of amyloid deposits increases dramatically between the ages of 10 and 12 months.

References
Original: J:80782 Borchelt DR et al., "A vector for expressing foreign genes in the brains and hearts of transgenic mice." Genet Anal 1996 Dec;13(6):159-63
All: 51 reference(s)
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11/20/2009
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