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Dffb Gene Detail
Summary
  • Symbol
    Dffb
  • Name
    DNA fragmentation factor, beta subunit
  • Synonyms
    40kDa, 5730477D02Rik, CAD, caspase-activated DNase, CPAN, DFF40, Didff
  • Feature Type
    protein coding gene
  • IDs
    MGI:1196287
    NCBI Gene: 13368
  • Alliance
    genome feature page
  • Transcription Start Sites
    2 TSS
Location &
Maps
more
  • Sequence Map
    Chr4:154048904-154059578 bp, - strand
    From NCBI annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 4, 83.78 cM, cytoband E2
  • Mapping Data
    2 experiments
Strain
Comparison
more
  • SNPs within 2kb
    443 from dbSNP Build 142
  • Strain Annotations
    19
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_1196287
 protein coding gene Chr4:154048904-154059583 (-)
129S1/SvImJ ENSMUSG00200018585
 protein coding gene Chr4:145110219-145120855 (-)
A/J ENSMUSG00195015205
 protein coding gene Chr4:146766962-146777665 (-)
AKR/J ENSMUSG00220024101
 protein coding gene Chr4:146353537-146364240 (-)
BALB/cJ ENSMUSG00180002217
 protein coding gene Chr4:144427417-144438124 (-)
C3H/HeJ ENSMUSG00175016827
 protein coding gene Chr4:147733884-147744587 (-)
C57BL/6NJ ENSMUSG00215007654
 protein coding gene Chr4:146564506-146575184 (-)
CAROLI/EiJ MGP_CAROLIEiJ_G0026862
 protein coding gene Chr4:143868422-143879967 (-)
CAST/EiJ ENSTCUG00005030762
 protein coding gene Chr4:150430649-150441072 (-)
CBA/J ENSMUSG00210005028
 protein coding gene Chr4:146392113-146402816 (-)
DBA/2J ENSMUSG00185016372
 protein coding gene Chr4:149949339-149960042 (-)
FVB/NJ ENSMUSG00205011621
 protein coding gene Chr4:144845947-144856650 (-)
JF1/MsJ ENSUMUG00000000220
 protein coding gene Chr4:152959661-152970035 (-)
LP/J ENSMUSG00230018687
 protein coding gene Chr4:156670541-156681248 (-)
NOD/ShiLtJ ENSMUSG00190003987
 protein coding gene Chr4:146049680-146060383 (-)
NZO/HlLtJ ENSMUSG00225023887
 protein coding gene Chr4:160665052-160675703 (-)
PWK/PhJ ENSLUMG00010026915
 protein coding gene Chr4:145224771-145235076 (-)
SPRET/EiJ ENSMSPG00010018589
 protein coding gene Chr4:147176112-147187978 (-)
WSB/EiJ ENSIUOG00005006354
 protein coding gene Chr4:144256545-144269959 (-)



Homology
more
  • Human Ortholog
    DFFB, DNA fragmentation factor subunit beta
  • Vertebrate Orthologs
    3
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    DFFB, DNA fragmentation factor subunit beta
  • Synonyms
    CAD, CPAN, DFF2, DFF-40, DFF40
  • Links
    NCBI Gene ID: 1677
    UniProt: O76075

  • Chr Location
    1p36.32; chr1:3857267-3885429 (+)  GRCh38
Mutations,
Alleles, and
Phenotypes
less
  • Phenotype Summary
    3 phenotypes from 2 alleles in 3 genetic backgrounds
    8 phenotypes from multigenic genotypes
    30 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
Mice homozygous for a knock-out allele are viable, fertile and developmentally normal; however, mutant thymocytes and other cell types fail to undergo apoptotic DNA fragmentation in response to dexamethasone or other apoptotic stimuli.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    45
  • GO References
    10
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
membraneless organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    597
  • cDNA Data
    23
  • Literature Summary
    2
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic 13368 NCBI Gene Model | MGI Sequence Detail 10675 C57BL/6J ±  kb
    transcript NM_007859 RefSeq | MGI Sequence Detail 2073 C57BL/6  
    polypeptide O54788 UniProt | EBI | MGI Sequence Detail 344 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    Molecular
    Reagents
    less
    • All nucleic 23
      cDNA 23

      Microarray probesets 5
    Other
    Accession IDs
    less
    MGI:2442077
    References
    more
    • Summaries
      All 73
      Developmental Gene Expression 2
      Gene Ontology 10
      Phenotypes 30
    • Earliest
      J:45015 Enari M, et al., A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD [see comments]. Nature. 1998 Jan 1;391(6662):43-50
    • Latest
      J:370814 Chen L, et al., EFTUD2 Regulates Cortical Morphogenesis via Modulation of Caspase-3 and Aifm1 Splicing Pathways. Adv Sci (Weinh). 2025 May 31;:e04200
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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    Funding Information
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    Send questions and comments to User Support.     		last database update
    09/08/2026
    MGI 6.24     		The Jackson Laboratory