Nefm MGI Mouse Gene Detail - MGI:97314 - neurofilament, medium polypeptide

Symbol

Nefm
Name

neurofilament, medium polypeptide
Synonyms

Nef3, neurofilament-M, NF160, NF165, Nfm, NF-M

Feature Type

protein coding gene
IDs

MGI:97314
NCBI Gene: 18040
Alliance

genome feature page
Transcription Start Sites

20 TSS

Sequence Map

Chr14:68356994-68362453 bp, - strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 14, 35.46 cM
Mapping Data

6 experiments

SNPs within 2kb

158 from dbSNP Build 142
Strain Annotations

19
RFLP

1

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_97314	protein coding gene	Chr14:68320039-68362453 (-)
129S1/SvImJ	ENSMUSG00200026351	protein coding gene	Chr14:56937615-56979867 (-)
A/J	ENSMUSG00195012864	protein coding gene	Chr14:57102501-57144741 (-)
AKR/J	ENSMUSG00220026741	protein coding gene	Chr14:56443114-56485348 (-)
BALB/cJ	ENSMUSG00180033834	protein coding gene	Chr14:56718653-56760893 (-)
C3H/HeJ	ENSMUSG00175030999	protein coding gene	Chr14:58474101-58516361 (-)
C57BL/6NJ	ENSMUSG00215012014	protein coding gene	Chr14:56655394-56697651 (-)
CAROLI/EiJ	MGP_CAROLIEiJ_G0019452	protein coding gene	Chr14:59229996-59274111 (-)
CAST/EiJ	ENSTCUG00005035112	protein coding gene	Chr14:58380341-58422046 (-)
CBA/J	ENSMUSG00210032497	protein coding gene	Chr14:57091135-57133366 (-)
DBA/2J	ENSMUSG00185023128	protein coding gene	Chr14:62271915-62314176 (-)
FVB/NJ	ENSMUSG00205021736	protein coding gene	Chr14:56605262-56647502 (-)
JF1/MsJ	ENSUMUG00000024976	protein coding gene	Chr14:73447356-73489619 (-)
LP/J	ENSMUSG00230016251	protein coding gene	Chr14:74301024-74343274 (-)
NOD/ShiLtJ	ENSMUSG00190039151	protein coding gene	Chr14:56884308-56926541 (-)
NZO/HlLtJ	ENSMUSG00225039349	protein coding gene	Chr14:64653993-64665860 (-)
PWK/PhJ	ENSLUMG00010033878	protein coding gene	Chr14:56327294-56369054 (-)
SPRET/EiJ	ENSMSPG00010028058	protein coding gene	Chr14:59823354-59862648 (-)
WSB/EiJ	ENSIUOG00005037112	protein coding gene	Chr14:56338694-56380785 (-)

Human Ortholog

NEFM, neurofilament medium chain

Vertebrate Orthologs

4

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

NEFM, neurofilament medium chain
Synonyms

NEF3, NF-M, NFM
Links

HGNC:7734

NCBI Gene ID: 4741

UniProt: P07197
Chr Location

8p21.2; chr8:24913758-24919098 (+) GRCh38

MGI Vertebrate Homology

Nefm stringent orthology
1 human;1 mouse;1 rat;2 zebrafish
HCOP

vertebrate homology predictions: NEFM
Gene Tree

Nefm

Diseases

2 with human NEFM associations

				Human Disease	Mouse Models
				Alzheimer's disease IDs Alzheimer's disease DOID:10652 ICD10CM:G30 ICD9CM:331.0 KEGG:05010 MESH:D000544 NCI:C2866 UMLS_CUI:C0002395
				human immunodeficiency virus infectious disease IDs human immunodeficiency virus infectious disease DOID:526 ICD10CM:B20 ICD9CM:042 ICD9CM:042-042.99 MESH:D015658 NCI:C3108 UMLS_CUI:C0019693

Click on a disease name to see all genes associated with that disease.

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Phenotype Summary

13 phenotypes from 4 alleles in 4 genetic backgrounds
13 phenotypes from multigenic genotypes
4 images
25 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

11
Chemically induced (other)

1
Endonuclease-mediated

1
Targeted

9
Genomic Mutations

1 involving Nefm
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

26 strains or lines available

Mice homozygous for a knock-out allele exhibit reduced axon diameter.

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All GO Annotations

41
GO References

24

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

3146
Tissues

542
cDNA Data

17
Literature Summary

853

Images

Tissue x Stage Matrix

Other Mouse Links

Allen Institute

GENSAT

GEO

Expression Atlas
Other Vertebrate Links

Xenbase nefm

ZFIN nefma, nefmb

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All Sequences

47
RefSeq

2
UniProt

3
CCDS

CCDS27233.1

Ensembl

ENSMUSG00000022054 (Evidence)
NCBI Gene

18040

			Length	Strain/Species	Flank
genomic	18040	NCBI Gene Model \| MGI Sequence Detail	5460	C57BL/6J	± kb
transcript	NM_008691	RefSeq \| MGI Sequence Detail	3244	C57BL/6
polypeptide	P08553	UniProt \| EBI \| MGI Sequence Detail	848	Not Applicable

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UniProt

3 Sequences
Protein Ontology

PR:000011120 neurofilament medium polypeptide

(term hierarchy)
InterPro Domains

IPR006821 Intermediate filament head, DNA-binding domain

IPR018039 Intermediate filament protein, conserved site

IPR039008 Intermediate filament, rod domain

IPR050405 Intermediate filament structural protein

IPR002957 Keratin, type I
GlyGen

P08553 20 sites, 1 O-linked glycan (20 sites)

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All nucleic 33

Genomic 4

cDNA 19

Primer pair 5

Other 5

Antibodies 79

Microarray probesets 5

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MGD-MRK-12832

Summaries

All 954

Developmental Gene Expression 853

Gene Ontology 24

Phenotypes 25
Earliest

J:11960 Julien JP, et al., Cloning and developmental expression of the murine neurofilament gene family. Brain Res Mol Brain Res. 1986 Dec;387(3):243-50
Latest

J:389900 Hernandez-Arce A, et al., The extracellular matrix in selective decussation of retinal ganglion cell axons: beta2 laminins regulate the ipsilateral projection. Dev Biol. 2026 Jul 21;

TSS for Nefm:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr129422	Chr14:68362285-68362305 (-)	158 bp
Tssr129421	Chr14:68361943-68361948 (-)	507 bp
Tssr129420	Chr14:68360335-68360362 (-)	2,104 bp
Tssr129419	Chr14:68358810-68358822 (-)	3,637 bp
Tssr129418	Chr14:68358768-68358783 (-)	3,677 bp
Tssr129417	Chr14:68358593-68358607 (-)	3,853 bp
Tssr129416	Chr14:68358530-68358539 (-)	3,918 bp
Tssr129415	Chr14:68358515-68358524 (-)	3,933 bp
Tssr129414	Chr14:68358422-68358434 (-)	4,025 bp
Tssr129413	Chr14:68358395-68358409 (-)	4,051 bp
Tssr129412	Chr14:68358338-68358349 (-)	4,109 bp
Tssr129411	Chr14:68358326-68358337 (-)	4,121 bp
Tssr129410	Chr14:68358264-68358291 (-)	4,175 bp
Tssr129409	Chr14:68358212-68358258 (-)	4,218 bp
Tssr129408	Chr14:68358158-68358176 (-)	4,286 bp
Tssr129407	Chr14:68357990-68358004 (-)	4,456 bp
Tssr129406	Chr14:68357937-68357948 (-)	4,510 bp
Tssr129405	Chr14:68357869-68357880 (-)	4,578 bp
Tssr129404	Chr14:68357737-68357760 (-)	4,704 bp
Tssr129403	Chr14:68357637-68357640 (-)	4,814 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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