Hoxd8 MGI Mouse Gene Detail - MGI:96209

Symbol

Hoxd8
Name

homeobox D8
Synonyms

4921540P06Rik, Hox-4.3, Hox-5.4

Feature Type

protein coding gene
IDs

MGI:96209
NCBI Gene: 15437
Member of

Hoxd cluster
Alliance

genome feature page
Transcription Start Sites

11 TSS
Regulated by

Rr31 (1 regulatory region)

Sequence Map

Chr2:74534973-74538277 bp, + strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 2, 44.13 cM, cytoband D
Mapping Data

6 experiments

SNPs within 2kb

93 from dbSNP Build 142
Strain Annotations

19
RFLP

1

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_96209	protein coding gene	Chr2:74534959-74538277 (+)
129S1/SvImJ	ENSMUSG00200002733	protein coding gene	Chr2:71583591-71586911 (+)
A/J	ENSMUSG00195019153	protein coding gene	Chr2:71730295-71733615 (+)
AKR/J	ENSMUSG00220002213	protein coding gene	Chr2:71694929-71698246 (+)
BALB/cJ	ENSMUSG00180006006	protein coding gene	Chr2:71683950-71687269 (+)
C3H/HeJ	ENSMUSG00175010441	protein coding gene	Chr2:71809846-71813165 (+)
C57BL/6NJ	ENSMUSG00215013112	protein coding gene	Chr2:71646791-71650109 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0023672	protein coding gene	Chr2:69509114-69512398 (+)
CAST/EiJ	ENSTCUG00005027605	protein coding gene	Chr2:71123705-71127010 (+)
CBA/J	ENSMUSG00210002011	protein coding gene	Chr2:71852501-71855819 (+)
DBA/2J	ENSMUSG00185010345	protein coding gene	Chr2:71688346-71691664 (+)
FVB/NJ	ENSMUSG00205013513	protein coding gene	Chr2:71140327-71143648 (+)
JF1/MsJ	ENSUMUG00000024130	protein coding gene	Chr2:71365522-71368807 (+)
LP/J	ENSMUSG00230024002	protein coding gene	Chr2:73483179-73486498 (+)
NOD/ShiLtJ	ENSMUSG00190009630	protein coding gene	Chr2:71763054-71766372 (+)
NZO/HlLtJ	ENSMUSG00225014193	protein coding gene	Chr2:81247245-81250563 (+)
PWK/PhJ	ENSLUMG00010030375	protein coding gene	Chr2:71603851-71607143 (+)
SPRET/EiJ	ENSMSPG00010019120	protein coding gene	Chr2:73003850-73007150 (+)
WSB/EiJ	ENSIUOG00005011322	protein coding gene	Chr2:71806327-71809645 (+)

Human Ortholog

HOXD8, homeobox D8

Vertebrate Orthologs

2

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

HOXD8, homeobox D8
Synonyms

HOX4, HOX4E, HOX5.4
Links

HGNC:5139

NCBI Gene ID: 3234

UniProt: P13378
Chr Location

2q31.1; chr2:176129694-176132695 (+) GRCh38

MGI Vertebrate Homology

Hoxd8 stringent orthology
1 human;1 mouse;1 rat
HCOP

vertebrate homology predictions: HOXD8
Gene Tree

Hoxd8

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Phenotype Summary

4 phenotypes from 1 allele in 1 genetic background
7 phenotypes from multigenic genotypes
50 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

33
Chemically induced (other)

1
Radiation induced

1
Spontaneous

1
Targeted

30
Genomic Mutations

32 involving Hoxd8
Incidental Mutations

Mutagenetix , APF
Find Mice (IMSR)

11 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Mice homozygous for a knock-out allele are viable and healthy but show minor and low penetrance homeotic transformations in both lumbar (L1 to T13) and thoracic (T8 to T7) vertebrae.

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All GO Annotations

28
GO References

8

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

951
Tissues

236
cDNA Data

16
Literature Summary

43

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

GEISHA HOXD8

Xenbase hoxd8

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All Sequences

47
RefSeq

6
UniProt

5
CCDS

CCDS16143.1, CCDS71077.1, CCDS79809.1

Ensembl

ENSMUSG00000027102 (Evidence)
NCBI Gene

15437

			Length	Strain/Species	Flank
genomic	15437	NCBI Gene Model \| MGI Sequence Detail	3305	C57BL/6J	± kb
transcript	NM_008276	RefSeq \| MGI Sequence Detail	2193	ZRU/MplStud
polypeptide	P23463	UniProt \| EBI \| MGI Sequence Detail	289	Not Applicable

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UniProt

5 Sequences
Protein Ontology

PR:000008723 homeobox protein Hox-D8

(term hierarchy)
InterPro Domains

IPR050948 Antp homeobox transcription factor

IPR017970 Homeobox, conserved site

IPR001827 Homeobox protein, antennapedia type, conserved site

IPR001356 Homeodomain

IPR009057 Homeodomain-like superfamily

IPR020479 Homeodomain, metazoa

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All nucleic 35

Genomic 8

cDNA 16

Primer pair 6

Other 5

Microarray probesets 3

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MGD-MRK-10850, MGD-MRK-10910, MGI:1913985, MGI:2138833

Summaries

All 117

Developmental Gene Expression 43

Gene Ontology 8

Phenotypes 50
Earliest

J:10491 Davisson MT, et al., The mouse mutation ulnaless on chromosome 2. J Hered. 1990 Mar-Apr;81(2):151-3
Latest

J:373797 Hintermann A, et al., Co-option of an ancestral cloacal regulatory landscape during digit evolution. Nature. 2025 Sep 17;

Hoxd8 expression regulated by:

Feature	Feature Type	Location	Reference
Rr31, regulatory region 31	enhancer	Chr2:75269157-75271345	J:199174

TSS for Hoxd8:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr18420	Chr2:74534876-74534889 (+)	-90 bp
Tssr18421	Chr2:74534967-74534992 (+)	7 bp
Tssr18422	Chr2:74535169-74535181 (+)	202 bp
Tssr18423	Chr2:74535205-74535258 (+)	259 bp
Tssr18424	Chr2:74535261-74535273 (+)	294 bp
Tssr18425	Chr2:74535390-74535422 (+)	433 bp
Tssr18426	Chr2:74535430-74535445 (+)	465 bp
Tssr18427	Chr2:74535489-74535500 (+)	522 bp
Tssr18428	Chr2:74535522-74535533 (+)	555 bp
Tssr18429	Chr2:74535684-74535722 (+)	730 bp
Tssr18430	Chr2:74536082-74536113 (+)	1,125 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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