En1
Gene Detail

Symbol Name ID

En1 engrailed 1 MGI:95389

STS

D1Mit217

Synonyms

En-1, engrailed-1, Mo-en.1

Feature Type

protein coding gene

Genetic Map

Chromosome 1

52.74 cM, cytoband C2-qter
Detailed Genetic Map ± 1 cM


Mapping data(24)

Sequence Map

Chr1:120602418-120609296 bp, + strand From VEGA annotation of GRCm38   6879 bp   ±  kb flank VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer

Mouse Genome Browser

Vertebrate homology

HomoloGene:50663  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 chimpanzee; 1 rhesus macaque; 1 cattle; 1 dog; 1 chicken; 1 zebrafish

Protein SuperFamily: homeobox protein, EN-1/EN-2 type
Gene Tree: En1

Human homologs

Human Homolog		EN1, engrailed homeobox 1
NCBI Gene ID		2019
neXtProt AC		NX_Q05925
Human Chr (Location)		2q14.2; chr2:119599747-119605759 (-)  GRCh37.p10

Alleles and phenotypes

All alleles(16) : Targeted(16)
 

Mutant homozygotes usually die within 24 hours of birth. Mutants exhibit nervous system defects, including a lack of most of the colliculi, cerebellum, and the third and fourth cranial nerves in some lines. Skeletal anomalies have also been described.

 
Alleles Annotated to Human Diseases(1)   

Gene Ontology (GO) classifications

All GO classifications: (21 annotations)

Process	dorsal/ventral pattern formation, embryonic forelimb morphogenesis, ...
Component	membrane, nucleus
Function	DNA binding, sequence-specific DNA binding, ...

External Resources: FuncBase

Expression

Literature Summary: (260 records)
Data Summary: Results (770)    Tissues (327)    Images (193)
Theiler Stages: 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28

Assay Type	Results
Immunohistochemistry	60
RNA in situ	546
In situ reporter (knock in)	67
Northern blot	42
RT-PCR	55

cDNA source data(27)
External Resources: Allen Institute   GENSAT   GEO   ArrayExpress

Molecular reagents

All nucleic(53) Genomic(6) cDNA(29) Primer pair(5) Other(13)
Microarray probesets(3)

Other database links

VEGA Gene Model	OTTMUSG00000037271 (Evidence)
Ensembl Gene Model	ENSMUSG00000058665 (Evidence)
Entrez Gene	13798 (Evidence)
UniGene	2657
DFCI	TC1589119
DoTS	DT.40167192
NIA Mouse Gene Index	U000729
Consensus CDS Project	CCDS15235.1
International Mouse Knockout Project Status	En1

Sequences

Length	Strain/Species
genomic	OTTMUSG00000037271	VEGA Gene Model | MGI Sequence Detail	6879	C57BL/6J
transcript	OTTMUST00000096137	VEGA | MGI Sequence Detail	3928	Not Applicable
polypeptide	OTTMUSP00000053676	VEGA | MGI Sequence Detail	401	Not Applicable

All sequences(22) RefSeq(2) UniProt(2)

Polymorphisms

RFLP(8) : SNPs(23 from dbSNP Build 128)

Protein-related information

Resource	ID	Description
InterPro	IPR017970	Homeobox, conserved site
InterPro	IPR019549	Homeobox engrailed, C-terminal
InterPro	IPR019737	Homeobox engrailed-type, conserved site
InterPro	IPR001356	Homeodomain
InterPro	IPR000747	Homeodomain engrailed
InterPro	IPR009057	Homeodomain-like
InterPro	IPR020479	Homeodomain, metazoa
Protein Ontology	PR:000007070	homeobox protein engrailed-1

References

(Earliest) J:8126 Joyner AL, et al., Expression during embryogenesis of a mouse gene with sequence homology to the Drosophila engrailed gene. Cell. 1985 Nov;43(1):29-37
(Latest) J:194842 Yang J, et al., Dynamic temporal requirement of Wnt1 in midbrain dopamine neuron development. Development. 2013 Mar;140(6):1342-52
All references(391)

Other accession IDs

MGD-MRK-12340, MGD-MRK-9497, MGD-MRK-9499