Spag4 MGI Mouse Gene Detail - MGI:2444120 - sperm associated antigen 4

Symbol

Spag4
Name

sperm associated antigen 4
Synonyms

1700041K21Rik, Sun4

Feature Type

protein coding gene
IDs

MGI:2444120
NCBI Gene: 245865
Alliance

gene page
Transcription Start Sites

3 TSS

Sequence Map

Chr2:155907108-155911421 bp, + strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 2, 77.34 cM
Mapping Data

1 experiment

SNPs within 2kb

74 from dbSNP Build 142
Strain Annotations

18

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_2444120	protein coding gene	Chr2:155907095-155912537 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0026754	protein coding gene	Chr2:160922841-160928302 (+)
A/J	MGP_AJ_G0026720	protein coding gene	Chr2:154536129-154541639 (+)
AKR/J	MGP_AKRJ_G0026691	protein coding gene	Chr2:159020726-159026168 (+)
BALB/cJ	MGP_BALBcJ_G0026729	protein coding gene	Chr2:154831070-154836524 (+)
C3H/HeJ	MGP_C3HHeJ_G0026472	protein coding gene	Chr2:159383927-159389381 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0027178	protein coding gene	Chr2:165652613-165658055 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0024548	protein coding gene	Chr2:148030773-148036023 (+)
CAST/EiJ	MGP_CASTEiJ_G0025933	protein coding gene	Chr2:159712057-159717472 (+)
CBA/J	MGP_CBAJ_G0026449	protein coding gene	Chr2:171659862-171676925 (+)
DBA/2J	MGP_DBA2J_G0026586	protein coding gene	Chr2:153708187-153713628 (+)
FVB/NJ	MGP_FVBNJ_G0026553	protein coding gene	Chr2:152087506-152093311 (+)
LP/J	MGP_LPJ_G0026691	protein coding gene	Chr2:161063092-161068558 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0026574	protein coding gene	Chr2:175625168-175630600 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0027234	protein coding gene	Chr2:158899511-158904939 (+)
PWK/PhJ	MGP_PWKPhJ_G0025667	protein coding gene	Chr2:153214669-153220107 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0025470	protein coding gene	Chr2:157844064-157849502 (+)
WSB/EiJ	MGP_WSBEiJ_G0026003	protein coding gene	Chr2:159786562-159791968 (+)

Human Ortholog

SPAG4, sperm associated antigen 4

Vertebrate Orthologs

2

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

SPAG4, sperm associated antigen 4
Synonyms

CT127, SUN4
Links

HGNC:11214

NCBI Gene ID: 6676

neXtProt AC: NX_Q9NPE6

UniProt: Q9NPE6
Chr Location

20q11.22; chr20:35615829-35621094 (+) GRCh38

MGI Vertebrate Homology

Spag4 stringent orthology
1 human;1 mouse;1 rat
HCOP

vertebrate homology predictions: SPAG4
Gene Tree

Spag4

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Phenotype Summary

21 phenotypes from 2 alleles in 3 genetic backgrounds
11 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

4
Gene trapped

1
Targeted

3
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

17 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Mice homozygous for a knock-out allele show disrupted spermiogenesis, severe defects in sperm head formation, abnormal manchette morphology, globozoospermia, and male infertility.

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All GO Annotations

10
GO References

4

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	non-membrane-bounded organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

714
Tissues

8
cDNA Data

11
Literature Summary

1

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas

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All Sequences

52
RefSeq

4
UniProt

7
CCDS

CCDS50777.1

Ensembl

ENSMUSG00000038180 (Evidence)
NCBI Gene

245865

			Length	Strain/Species	Flank
genomic	245865	NCBI Gene Model \| MGI Sequence Detail	4314	C57BL/6J	± kb
transcript	NM_139151	RefSeq \| MGI Sequence Detail	1502	C57BL/6
polypeptide	Q9JJF2	UniProt \| EBI \| MGI Sequence Detail	443	Not Applicable

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UniProt

7 Sequences
Protein Ontology

PR:000015457 sperm-associated antigen 4 protein

(term hierarchy)
InterPro Domains

IPR012919 SUN domain

IPR045119 SUN domain-containing protein 1-5

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All nucleic 12

Genomic 1

cDNA 11

Microarray probesets 3

Summaries

All 33

Developmental Gene Expression 1

Gene Ontology 4

Phenotypes 11
Earliest

J:80000 The FANTOM Consortium and The RIKEN Genome Exploration Research Group Phase I & II Team, Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature. 2002;420:563-573
Latest

J:339229 Thoma H, et al., SUN4 is a spermatid type II inner nuclear membrane protein that forms heteromeric assemblies with SUN3 and interacts with lamin B3. J Cell Sci. 2023 Apr 1;136(7)

TSS for Spag4:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr21138	Chr2:155907100-155907202 (+)	43 bp
Tssr21139	Chr2:155907649-155907656 (+)	545 bp
Tssr21140	Chr2:155907658-155907680 (+)	561 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23