Dock4 Gene Detail

Summary

• Symbol
  Dock4
• Name
  dedicator of cytokinesis 4
• Synonyms
  6330411N01Rik, EST N28122, mKIAA0716

• Feature Type
  protein coding gene
• IDs
  MGI:1918006
  NCBI Gene: 238130
• Alliance
  gene page
• Transcription Start Sites
  34 TSS
• Candidate for QTL
  1 QTL

Location &
Maps

• Sequence Map
  Chr12:40495956-40896873 bp, + strand

  From NCBI annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 12, 18.06 cM
• Mapping Data
  3 experiments

Strain
Comparison

• SNPs within 2kb
  24752 from dbSNP Build 142
• Strain Annotations
  18
• PCR
  1

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1918006	protein coding gene	Chr12:40495951-40896873 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0019705	protein coding gene	Chr12:38863099-39280689 (+)
A/J	MGP_AJ_G0019671	protein coding gene	Chr12:37587615-37994829 (+)
AKR/J	MGP_AKRJ_G0019642	protein coding gene	Chr12:38729932-39136356 (+)
BALB/cJ	MGP_BALBcJ_G0019649	protein coding gene	Chr12:37800211-38203840 (+)
C3H/HeJ	MGP_C3HHeJ_G0019453	protein coding gene	Chr12:38657588-39077611 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0020096	protein coding gene	Chr12:40021859-40440724 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0017721	protein coding gene	Chr12:36707188-37112752 (+)
CAST/EiJ	MGP_CASTEiJ_G0019006	protein coding gene	Chr12:33834831-34275452 (+)
CBA/J	MGP_CBAJ_G0019425	protein coding gene	Chr12:41384851-41815284 (+)
DBA/2J	MGP_DBA2J_G0019538	protein coding gene	Chr12:37438238-37840317 (+)
FVB/NJ	MGP_FVBNJ_G0019527	protein coding gene	Chr12:37067772-37470025 (+)
LP/J	MGP_LPJ_G0019608	protein coding gene	Chr12:38860225-39281084 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0019563	protein coding gene	Chr12:41531570-41969638 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0020136	protein coding gene	Chr12:38289186-38706299 (+)
PWK/PhJ	MGP_PWKPhJ_G0018773	protein coding gene	Chr12:32113309-32523133 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0018563	protein coding gene	Chr12:33126539-33365683 (+)
WSB/EiJ	MGP_WSBEiJ_G0019056	protein coding gene	Chr12:38592250-39008377 (+)

Homology

• Human Ortholog
  DOCK4, dedicator of cytokinesis 4

• Vertebrate Orthologs
  3

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  DOCK4, dedicator of cytokinesis 4
  
• Links
  HGNC:19192

  NCBI Gene ID: 9732

  UniProt: Q8N1I0

  
  
• Chr Location
  7q31.1; chr7:111726110-112206407 (-)  GRCh38

---

• MGI Vertebrate Homology
  Dock4 stringent orthology
  1 human;1 mouse;1 rat;1 zebrafish
  
• HCOP
  vertebrate homology predictions: DOCK4
• Gene Tree
  Dock4
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  6 phenotypes from 2 alleles in 2 genetic backgrounds
  1 images
  13 phenotype references
  

• All Mutations and Alleles
  11
• Endonuclease-mediated
  2
• Gene trapped
  5
• Targeted
  4
• Incidental Mutations
  Mutagenetix , APF , CvDC
• Find Mice (IMSR)
  108 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Homozygous disruption of this gene leads to complete embryonic lethality. Heterozygotes display altered blood vessel lumen formation.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  52
• GO References
  10

Expression

• Assay Results
  712
• Tissues
  5
• cDNA Data
  37
• Literature Summary
  3

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  Xenbase dock4
  ZFIN dock4a, dock4b

Sequences &
Gene Models

• All Sequences
  74
• RefSeq
  24
• UniProt
  5
• CCDS
  CCDS25895.1

• Ensembl
  ENSMUSG00000035954 (Evidence)
• NCBI Gene
  238130

Representative Sequences			Length	Strain/Species
genomic	238130	NCBI Gene Model | MGI Sequence Detail	400918	C57BL/6J
transcript	NM_172803	RefSeq | MGI Sequence Detail	8072	Not Specified
polypeptide	P59764	UniProt | EBI | MGI Sequence Detail	1978	Not Applicable

Protein
Information

• UniProt
  5 Sequences
• Protein Ontology
  PR:000006621 dedicator of cytokinesis protein 4
  (term hierarchy)
• InterPro Domains
  IPR016024 Armadillo-type fold
  IPR027007 C2 DOCK-type domain
  IPR035892 C2 domain superfamily
  IPR026791 Dedicator of cytokinesis
  IPR035769 Dedicator of cytokinesis 4, SH3 domain
  IPR037811 Dedicator of cytokinesis B, C2 domain
  IPR043161 Dedicator of cytokinesis, C-terminal, lobe A
  IPR043162 Dedicator of cytokinesis, C-terminal, lobe C
  IPR032376 Dedicator of cytokinesis, N-terminal domain
  IPR042455 Dedicator of cytokinesis, N-terminal, subdomain 1
  IPR037014 Dedicator of cytokinesis protein 4, DHR2 domain
  IPR027357 DOCKER domain
  IPR046769 DOCKER, Lobe A
  IPR046770 DOCKER, Lobe B
  IPR046773 DOCKER, Lobe C
  IPR001452 SH3 domain
  IPR036028 SH3-like domain superfamily
• GlyGen
  P59764 5 sites, 1 O-linked glycan (3 sites)

Molecular
Reagents

• All nucleic 47

  cDNA 45

  Primer pair 2

  
  

  Microarray probesets 2

Other
Accession IDs

MGI:1858606

References

• Summaries
  All 51

  Developmental Gene Expression 3

  Gene Ontology 10

  Phenotypes 13
• Earliest
  J:62157 Thomas JW, et al., Comparative genome mapping in the sequence-based era: early experience with human chromosome 7. Genome Res. 2000 May;10(5):624-33
• Latest
  J:345621 Adams DJ, et al., Genetic determinants of micronucleus formation in vivo. Nature. 2024 Mar;627(8002):130-136

TSS for Dock4:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr113310	Chr12:40495992-40496014 (+)	47 bp
Tssr113311	Chr12:40496049-40496098 (+)	118 bp
Tssr3829	Chr12:40496118-40496127 (+)	167 bp
Tssr113312	Chr12:40496147-40496158 (+)	197 bp
Tssr113313	Chr12:40501024-40501060 (+)	5,086 bp
Tssr113314	Chr12:40505033-40505037 (+)	9,079 bp
Tssr113315	Chr12:40511120-40511137 (+)	15,173 bp
Tssr113316	Chr12:40515177-40515201 (+)	19,233 bp
Tssr113317	Chr12:40515211-40515225 (+)	19,262 bp
Tssr113318	Chr12:40515648-40515671 (+)	19,704 bp
Tssr113319	Chr12:40515726-40515768 (+)	19,791 bp
Tssr113320	Chr12:40522717-40522732 (+)	26,769 bp
Tssr113321	Chr12:40522866-40522870 (+)	26,912 bp
Tssr113322	Chr12:40530756-40530773 (+)	34,809 bp
Tssr113323	Chr12:40554181-40554185 (+)	58,227 bp
Tssr113324	Chr12:40568909-40568935 (+)	72,966 bp
Tssr113325	Chr12:40579620-40579625 (+)	83,667 bp
Tssr113326	Chr12:40588837-40588842 (+)	92,884 bp
Tssr113327	Chr12:40605699-40605712 (+)	109,750 bp
Tssr113328	Chr12:40606241-40606254 (+)	110,292 bp
Tssr113329	Chr12:40610239-40610259 (+)	114,293 bp
Tssr113330	Chr12:40610264-40610278 (+)	114,315 bp
Tssr113331	Chr12:40610389-40610400 (+)	114,439 bp
Tssr113332	Chr12:40622803-40622810 (+)	126,851 bp
Tssr113333	Chr12:40622830-40622844 (+)	126,881 bp
Tssr113334	Chr12:40625615-40625617 (+)	129,660 bp
Tssr113335	Chr12:40652272-40652305 (+)	156,333 bp
Tssr113336	Chr12:40698279-40698288 (+)	202,328 bp
Tssr113337	Chr12:40701989-40702009 (+)	206,043 bp
Tssr113338	Chr12:40745500-40745503 (+)	249,546 bp
Tssr113339	Chr12:40762041-40762047 (+)	266,088 bp
Tssr113340	Chr12:40768374-40768377 (+)	272,420 bp
Tssr113341	Chr12:40844258-40844270 (+)	348,308 bp
Tssr113342	Chr12:40844283-40844297 (+)	348,334 bp

Dock4 is Candidate Gene for:

QTL	Genetic Location*	Genome Location (GRCm39)	Reference	QTL Note
Ath6	Chr12, syntenic	Chr12:15758613-15758756	J:109430	It is believed that the enhanced diabetes susceptibility of C57BLKS/J-Lepr animals (compared to C57BL/6J-Lepr animals) is conferred by contribution of DBA/2J-derived DNA in the genome. It is estimated that 70% of the C57BLKS/J genome is of C57BL/6J origin, 20% is of DBA/2J origin, and 9% contributed by an unknown donor strain. Extensive SNP analysis using 15,300 SNPs at an average spacing of 167,000 bp on the autosomes and 330,000 bp on the X chromosome mapped blocks of C57BLKS/J genome derived from DBA/2J. In addition, expression analysis of genes in these blocks identified potential diabetes susceptibility candidate genes. Previously identified QTL Ath6 at 3 cM on mouse Chromosome 12 mapped to one of these DBA/2J-derived blocks from 10.4 Mb to 19.5 Mb. Over 430 genes are found in this interval. Genes exhibiting significant expression differences between C57BL/6J and DBA/2J and correlating with diabetes-related traits were screened. Rrm2 (8 cM; 19.15 Mb; LOD=13.92), Dock4 (16.5 cM; 35.35 Mb; LOD=8.75), and Cfl2 (36.5 cM; 50.275 Mb; LOD=10.33) were identified as Ath6 candidate genes meeting the above criteria.

*cM position of peak correlated region/marker