Pus3 MGI Mouse Gene Detail - MGI:1914299 - pseudouridine synthase 3

Symbol

Pus3
Name

pseudouridine synthase 3
Synonyms

2610020J05Rik, 5730412F04Rik

Feature Type

protein coding gene
IDs

MGI:1914299
NCBI Gene: 67049
Alliance

genome feature page
Transcription Start Sites

1 TSS

Sequence Map

Chr9:35469891-35478697 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 9, 20.32 cM, cytoband A5.3
Mapping Data

3 experiments

SNPs within 2kb

232 from dbSNP Build 142
Strain Annotations

19

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1914299	protein coding gene	Chr9:35469856-35478697 (+)
129S1/SvImJ	ENSMUSG00200037655	protein coding gene	Chr9:32448870-32459395 (+)
A/J	ENSMUSG00195046469	protein coding gene	Chr9:32240248-32250823 (+)
AKR/J	ENSMUSG00220045504	protein coding gene	Chr9:32280694-32291274 (+)
BALB/cJ	ENSMUSG00180042614	protein coding gene	Chr9:32368566-32379141 (+)
C3H/HeJ	ENSMUSG00175045365	protein coding gene	Chr9:32369749-32380105 (+)
C57BL/6NJ	ENSMUSG00215027242	protein coding gene	Chr9:32369435-32380011 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0031957	protein coding gene	Chr9:31192197-31201222 (+)
CAST/EiJ	ENSTCUG00005043008	protein coding gene	Chr9:32340580-32351167 (+)
CBA/J	ENSMUSG00210043983	protein coding gene	Chr9:32380677-32391258 (+)
DBA/2J	ENSMUSG00185036341	protein coding gene	Chr9:32420674-32431254 (+)
FVB/NJ	ENSMUSG00205031627	protein coding gene	Chr9:32735299-32745874 (+)
JF1/MsJ	ENSUMUG00000039149	protein coding gene	Chr9:32604905-32615465 (+)
LP/J	ENSMUSG00230029247	protein coding gene	Chr9:32844974-32855534 (+)
NOD/ShiLtJ	ENSMUSG00190021013	protein coding gene	Chr9:32498157-32508737 (+)
NZO/HlLtJ	ENSMUSG00225020622	protein coding gene	Chr9:32813009-32823569 (+)
PWK/PhJ	ENSLUMG00010038911	protein coding gene	Chr9:32486292-32496857 (+)
SPRET/EiJ	ENSMSPG00010032280	protein coding gene	Chr9:32912470-32922269 (+)
WSB/EiJ	ENSIUOG00005041265	protein coding gene	Chr9:31942061-31952633 (+)

Human Ortholog

PUS3, pseudouridine synthase 3

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

PUS3, pseudouridine synthase 3
Synonyms

2610020J05Rik, DEG1, FKSG32, MRT55, NEDMIGS
Links

HGNC:25461

NCBI Gene ID: 83480

UniProt: Q9BZE2
Chr Location

11q24.2; chr11:125893485-125903224 (-) GRCh38

MGI Vertebrate Homology

Pus3 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: PUS3
Gene Tree

Pus3

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Phenotype Summary

4 phenotypes from 1 allele in 1 genetic background
11 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

38
Endonuclease-mediated

3
Gene trapped

33
Targeted

2
Incidental Mutations

Mutagenetix , APF
Find Mice (IMSR)

20 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

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All GO Annotations

22
GO References

9

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

699
Tissues

10
cDNA Data

11
Literature Summary

1

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase pus3

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All Sequences

45
RefSeq

2
UniProt

4
CCDS

CCDS22965.1

Ensembl

ENSMUSG00000032103 (Evidence)
NCBI Gene

67049

			Length	Strain/Species	Flank
genomic	ENSMUSG00000032103	Ensembl Gene Model \| MGI Sequence Detail	8807	C57BL/6J	± kb
transcript	ENSMUST00000034615	Ensembl \| MGI Sequence Detail	2019	Not Applicable
polypeptide	ENSMUSP00000034615	Ensembl \| MGI Sequence Detail	481	Not Applicable

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UniProt

4 Sequences
Protein Ontology

PR:000013498 tRNA pseudouridine synthase 3

(term hierarchy)
EC

5.4.99.45
InterPro Domains

IPR020103 Pseudouridine synthase, catalytic domain superfamily

IPR001406 Pseudouridine synthase I, TruA

IPR020097 Pseudouridine synthase I, TruA, alpha/beta domain

IPR020095 Pseudouridine synthase I, TruA, C-terminal

IPR041707 Pseudouridine synthase Pus3-like

IPR020094 Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal

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All nucleic 11

cDNA 11

Microarray probesets 6

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MGI:1917733

Summaries

All 39

Developmental Gene Expression 1

Gene Ontology 9

Phenotypes 11
Earliest

J:85114 Chen J, et al., Pseudouridine synthase 3 from mouse modifies the anticodon loop of tRNA. Biochemistry. 2000 Oct 17;39(41):12723-30
Latest

J:326541 Cyagen Biosciences Inc., Cyagen Biosciences Website. 2022;

TSS for Pus3:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr84350	Chr9:35470756-35470789 (+)	882 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/08/2026 MGI 6.24