Spo11 MGI Mouse Gene Detail - MGI:1349669 - SPO11 initiator of meiotic double stranded breaks

Symbol

Spo11
Name

SPO11 initiator of meiotic double stranded breaks
Synonyms

Spo11a, Spo11b

Feature Type

protein coding gene
IDs

MGI:1349669
NCBI Gene: 26972
Alliance

gene page
Transcription Start Sites

12 TSS

Sequence Map

Chr2:172819493-172835369 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 2, 95.64 cM, cytoband H4
Mapping Data

5 experiments

SNPs within 2kb

808 from dbSNP Build 142
Strain Annotations

18
RFLP

2

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1349669	protein coding gene	Chr2:172819172-172835369 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0026950	protein coding gene	Chr2:179216876-179234530 (+)
A/J	MGP_AJ_G0026917	protein coding gene	Chr2:171684655-171700532 (+)
AKR/J	MGP_AKRJ_G0026888	protein coding gene	Chr2:176858847-176877746 (+)
BALB/cJ	MGP_BALBcJ_G0026926	protein coding gene	Chr2:172084539-172100744 (+)
C3H/HeJ	MGP_C3HHeJ_G0026668	protein coding gene	Chr2:177242280-177258148 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0027373	protein coding gene	Chr2:184371378-184388638 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0024743	protein coding gene	Chr2:164926283-164941557 (+)
CAST/EiJ	MGP_CASTEiJ_G0026126	protein coding gene	Chr2:177939382-177956687 (+)
CBA/J	MGP_CBAJ_G0026645	protein coding gene	Chr2:191278053-191293962 (+)
DBA/2J	MGP_DBA2J_G0026783	protein coding gene	Chr2:170781698-170797595 (+)
FVB/NJ	MGP_FVBNJ_G0026749	protein coding gene	Chr2:169399045-169415048 (+)
LP/J	MGP_LPJ_G0026884	protein coding gene	Chr2:179154985-179172263 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0026768	protein coding gene	Chr2:194222234-194238066 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0027430	protein coding gene	Chr2:176872998-176888697 (+)
PWK/PhJ	MGP_PWKPhJ_G0025863	protein coding gene	Chr2:170573470-170589495 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0025663	protein coding gene	Chr2:175864870-175882914 (+)
WSB/EiJ	MGP_WSBEiJ_G0026199	protein coding gene	Chr2:177830068-177845738 (+)

Human Ortholog

SPO11, SPO11 initiator of meiotic double strand breaks

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

SPO11, SPO11 initiator of meiotic double strand breaks
Synonyms

CT35, SPATA43, TOPOVIA, TOPVIA
Links

HGNC:11250

NCBI Gene ID: 23626

UniProt: Q9Y5K1
Chr Location

20q13.31; chr20:57329803-57343994 (+) GRCh38

MGI Vertebrate Homology

Spo11 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: SPO11
Gene Tree

Spo11

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Phenotype Summary

41 phenotypes from 8 alleles in 12 genetic backgrounds
15 phenotypes from multigenic genotypes
4 images
107 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

19
Endonuclease-mediated

6
Targeted

12
Transgenic

1
Incidental Mutations

APF
Find Mice (IMSR)

21 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Homozygous knockout affects double-stranded breaks in male meiosis causing loss of spermatocytes in early prophase and sterility, while mutant females exhibit oocyte loss soon after birth.

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All GO Annotations

40
GO References

17

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

880
Tissues

37
cDNA Data

17
Literature Summary

30

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase spo11

ZFIN spo11

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All Sequences

73
RefSeq

24
UniProt

2
CCDS

CCDS17137.1, CCDS50811.1, CCDS50812.1

Ensembl

ENSMUSG00000005883 (Evidence)
NCBI Gene

26972

			Length	Strain/Species	Flank
genomic	ENSMUSG00000005883	Ensembl Gene Model \| MGI Sequence Detail	15877	C57BL/6J	± kb
transcript	ENSMUST00000050442	Ensembl \| MGI Sequence Detail	1709	Not Applicable
polypeptide	ENSMUSP00000059056	Ensembl \| MGI Sequence Detail	396	Not Applicable

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UniProt

2 Sequences
Protein Ontology

PR:000015553 meiotic recombination protein SPO11

(term hierarchy)
EC

5.6.2.2
InterPro Domains

IPR004084 Meiosis-specific protein Spo11

IPR013048 Meiotic recombination, Spo11

IPR002815 Spo11/DNA topoisomerase VI subunit A

IPR013049 Spo11/DNA topoisomerase VI, subunit A, N-terminal

IPR036078 Spo11/DNA topoisomerase VI subunit A superfamily

IPR034136 Topoisomerase 6 subunit A/Spo11, TOPRIM domain

IPR036388 Winged helix-like DNA-binding domain superfamily

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All nucleic 32

Genomic 2

cDNA 17

Primer pair 11

Other 2

Microarray probesets 3

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MGI:2138956

Summaries

All 165

Developmental Gene Expression 30

Gene Ontology 17

Phenotypes 107
Earliest

J:58262 Romanienko PJ, et al., Cloning, characterization, and localization of mouse and human SPO11. Genomics. 1999 Oct 15;61(2):156-69
Latest

J:367782 Tang X, et al., In vitro reconstitution of meiotic DNA double-strand-break formation. Nature. 2025 Mar;639(8055):800-807

TSS for Spo11:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr21978	Chr2:172819346-172819351 (+)	-144 bp
Tssr21979	Chr2:172819418-172819425 (+)	-71 bp
Tssr21980	Chr2:172819446-172819469 (+)	-35 bp
Tssr21981	Chr2:172819475-172819488 (+)	-11 bp
Tssr21982	Chr2:172819493-172819504 (+)	6 bp
Tssr21983	Chr2:172819524-172819544 (+)	41 bp
Tssr21984	Chr2:172819551-172819582 (+)	74 bp
Tssr21985	Chr2:172819645-172819656 (+)	158 bp
Tssr21986	Chr2:172819668-172819694 (+)	188 bp
Tssr21987	Chr2:172819701-172819715 (+)	215 bp
Tssr21988	Chr2:172821620-172821672 (+)	2,153 bp
Tssr21989	Chr2:172834510-172834512 (+)	15,018 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24