Microarray analysis was used to identify genes showing alcohol-induced differential expression between inbred strains C57BL/6J and DBA/2J. These genes were then correlated to 6 previously identified alcohol-related QTLs to identify potential candidate genes. Two different Affymetrix microarrays were used in the analysis. Brain expression data from parental strains and 56 (DBA/2J x C57BL/6J)F2 intercross animals were used for analysis.
On mouse Chromosome 1, several candidate genes were identified for Alcw1 at 95.8 cM, Alcdp1 (95.8 cM), and Actre1 (85 cM). The highest priority candidate genes identified for chromosome 1 ethanol-related QTLs are Myoc (83.6 cM), Pou2f1 (87.2 cM), Mgst3, Aldh9a1, Sdhc, Kcnj9 (94.2 cM), Darc (94 cM), and Srp9. Other candidate genes with a ranking of modest priority were identified but are not listed here.