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| Nomenclature |
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Symbol:
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Gt(ROSA)26Sortm4(HIF2A*)Kael
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Name:
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gene trap ROSA 26, Philippe Soriano;
targeted mutation 4, William G Kaelin
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MGI ID: |
MGI:3832386 |
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Synonyms: |
HIF2dPA, LSL-HIF2dPA, ROSA26-HA-HIF2dPA |
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Gene:
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Gt(ROSA)26Sor
Location:
Chr6:113067428-113077333 bp, - strand
Genetic Position: Chr6,
52.73 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:144666
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Parent Cell Line:
| TC1/TC-1 (ES Cell) |
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Strain of Origin:
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129S6/SvEvTac
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutations: | |
Insertion, Nucleotide substitutions |
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Mutation details: DNA encoding influenza virus hemagglutinin A- (HA-) tagged human hypoxia-inducible factor 2 alpha (HIF2alpha) with nucleotide substitutions leading to replacement of proline by alanine at amino acid positions 405 and 531 (P405A;P531A), preceded by a floxed neo-STOP cassette, was inserted into the targeted locus. The STOP sequence must be removed by Cre-mediated recombination to allow for expression of the mutated coding sequence. The amino acid substitutions prevent oxygen-dependent prolyl hydroxylation at a.a. position 405 or 531, which is required for binding by von Hippel-Lindau tumor suppressor (VHL). (J:144666)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:144666
Kim WY et al.,
"Failure to prolyl hydroxylate hypoxia-inducible factor alpha phenocopies VHL inactivation in vivo."
EMBO J 2006 Oct 4;25(19):4650-62
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All: |
3 reference(s)
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Analysis Tools
