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| Nomenclature |
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Symbol:
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Snord116tm1.1Uta
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Name:
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small nucleolar RNA, C/D box 116 cluster;
targeted mutation 1.1, Uta Francke
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MGI ID: |
MGI:3773673 |
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Synonyms: |
1-lox, 1-loxp, Snord116del |
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Gene:
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Snord116
Location:
unknown
Genetic Position: Chr7,
Syntenic
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:131427
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Parent Cell Line:
| Bruce 4 (ES Cell) |
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Strain of Origin:
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B6.Cg-Thy1a
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutation: | |
Deletion |
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Mutation details: Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts. (J:131427)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:131427
Ding F et al.,
"SnoRNA Snord116 (Pwcr1/MBII-85) Deletion Causes Growth Deficiency and Hyperphagia in Mice."
PLoS ONE 2008;3(3):e1709
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All: |
2 reference(s)
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Analysis Tools
