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| Nomenclature |
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Symbol:
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Braftm1Mmcm
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Name:
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Braf transforming gene;
targeted mutation 1, Martin McMahon
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MGI ID: |
MGI:3711771 |
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Synonyms: |
BrafCA, BRAFF-V600E |
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Gene:
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Braf
Location:
Chr6:39603237-39725463 bp, - strand
Genetic Position: Chr6,
18.43 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:121715
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Parent Cell Line:
| E14 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: A targeting vector was designed to insert a loxP site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a floxed neo cassette. A modified exon 15 was engineered into the vector to encode Braf and a silent XbaI restriction site. RT-PCR confirmed that mutant transcript was expressed at the same level as wild-type, and that the V600E mutation was not detected. (J:121715)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:121715
Dankort D et al.,
"A new mouse model to explore the initiation, progression, and therapy of BRAFV600E-induced lung tumors."
Genes Dev 2007 Feb 15;21(4):379-84
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All: |
16 reference(s)
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Analysis Tools
