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| Nomenclature |
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Symbol:
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Gt(ROSA)26Sortm1(rtTA*M2)Jae
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Name:
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gene trap ROSA 26, Philippe Soriano;
targeted mutation 1, Rudolf Jaenisch
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MGI ID: |
MGI:3702294 |
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Synonyms: |
Gt(ROSA)26Sortm1(M2rtTA)Jae, R26-M2rtTA, R26-rtTA, Rosa26-rtRA-nls |
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Gene:
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Gt(ROSA)26Sor
Location:
Chr6:113067428-113077333 bp, - strand
Genetic Position: Chr6,
52.73 cM
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Col1a1tm1(tetO-Tcfap2c)Hsc/Col1a1+ Gt(ROSA)26Sortm1(rtTA*M2)Jae/Gt(ROSA)26Sor+ mice show increased proliferation and induced hepatic steatosis
Show the 4 image(s) involving this allele.
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:98920
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Parent Cell Line:
| v6.5 (ES Cell) |
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Strain of Origin:
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(C57BL/6 x 129S4/SvJae)F1
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivatory protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. (J:98920)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
This mutation was originally created downstream of the Col1a1tm2(tetO-Pou5f1)Jae allele in mutant ES cell line KH2 but the two mutations have subsequently been bred apart.
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| References |
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Original: |
J:98920
Hochedlinger K et al.,
"Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues."
Cell 2005 May 6;121(3):465-77
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All: |
42 reference(s)
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Analysis Tools
