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| Nomenclature |
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Symbol:
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Polhtm1Crey
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Name:
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polymerase (DNA directed), eta (RAD 30 related);
targeted mutation 1, Claude-Agnes Reynaud
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MGI ID: |
MGI:3577345 |
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Synonyms: |
Polh- |
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Gene:
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Polh
Location:
Chr17:46172004-46202625 bp, - strand
Genetic Position: Chr17,
22.9 cM, cytoband C
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:98037
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Parent Cell Line:
| E14.1 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutation: | |
Intragenic deletion |
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Mutation details: LoxP sites were introduced to flank exon 4, which contains the DE polymerase motifs conserved in all Y family polymerases. Cre expression mediated excision of exon 4 in ES cells, resulting in the direct splicing of exons 3-5. The out-of-frame junction would introduce a stop codon and therefore a truncated protein of 102 amino acids. (J:98037)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:98037
Delbos F et al.,
"Contribution of DNA polymerase {eta} to immunoglobulin gene hypermutation in the mouse."
J Exp Med 2005 Apr 18;201(8):1191-6
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All: |
3 reference(s)
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Analysis Tools
