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| Nomenclature |
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Symbol:
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Apctm1Tno
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Name:
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adenomatosis polyposis coli;
targeted mutation 1, Tetsuo Noda
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MGI ID: |
MGI:1857966 |
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Synonyms: |
580S, Apc580s, Apc580S flox, Apcf, Apcfl, ApcloxP |
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Gene:
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Apc
Location:
Chr18:34220924-34318608 bp, + strand
Genetic Position: Chr18,
18.53 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:43301
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Parent Cell Line:
| J1 (ES Cell) |
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Strain of Origin:
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129S4/SvJae
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutation: | |
Insertion |
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Mutation details: One loxP site was inserted into intron 13 and the other, with a PGK-neo cassette, into intron 14. Northern blot analysis revealed that RNA expression in the intestinal tract of homozygous mutants was about 30% of that in wild-type mice. Protein expression in the colon of homozygotes by immunohistochemistry was indistinguishable from that of wild-type mice. Western blot analysis of lysates from mouse embryo fibroblasts showed reduced expression of the protein. (J:43301, J:156864)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Apc Mutation:
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50 strains or lines available |
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| References |
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Original: |
J:43301
Shibata H et al.,
"Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene."
Science 1997 Oct 3;278(5335):120-3
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All: |
55 reference(s)
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Analysis Tools
