Phenotype Image Detail

Image

Caption

Rhombomere-specific deletion of commissures. Coronal sections at the level of r3, r5, and r6 of E11 control (A-C') or Robo3^tm1.1Ache/Robo3^tm1.1Ache Egr2^tm2(cre)Pch/Egr2⁺ (Krox20::cre;Robo3^lox/lox) (D-F') embryos immunostained with antibodies against Robo3, neurofilament, and Hb9. The midline is indicated by an arrow, and the Hb9-positive abducens motor neurons by an asterisk. In r3 and r5, there is an almost complete absence of Robo3 and neurofilament-positive commissural axons in mutant (D-E') compared to controls (A-B'). The density of neurofilament-positive axons is also strongly reduced. By contrast, there is no obvious reduction of commissural axons or Robo3 expression in r6 (C, C', F, and F'). Note that at E11, abducens shape and position are not altered in mutants compared to controls. VII, migrating facial neurons and facial nerve. Scale bars represent 60 um.

Copyright

This image is from Renier N, PLoS Biol 2010;8(3):e1000325, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:158918

Associated Alleles

Symbol	Name
Egr2^tm2(cre)Pch	early growth response 2; targeted mutation 2, Patrick Charnay
Robo3^tm1.1Ache	roundabout guidance receptor 3; targeted mutation 1.1, Alain Chedotal

Associated Genotypes

Allelic Composition	Genetic Background
Egr2^tm2(cre)Pch/Egr2^+ Robo3^tm1.1Ache/Robo3^tm1.1Ache	involves: 129S2/SvPas