Phenotype Image Detail

Image

Caption

Trio-deleted cerebellar external granule cell layer (EGL) microexplant in culture displayed impaired formation of neurites. Expression of Tuj1 (A and B), Tau (C, D, and D'), and TAG-1 (E, F, and F') in P4 EGL microexplants 36 h after culture. The dashed lines indicate the original border of the explants. D' and F' are magnifications of D and F, respectively. Trio^tm1Mzhu/Trio^tm1Mzhu Tg(Nes-cre)1Kln/0 (Trio^NKO) microexplant had short, disoriented, and fewer axons (B, D, and D') and virtually no long parallel fiber formation (TAG-1 staining) (F and F'). G, microexplants were prepared from P4 EGL and cultured for 30 h before imaging. Explants are at the bottom of the frame. Intervals between pictures were 60 min. The control neuron extended long radial axons under the guidance of growth cones (arrows). The mutant neuron (asterisk) extended short axons with many branches (arrowhead). Frequently, the extended axon may retract (long dashed arrow) and then re-extend again (short dashed arrow). Scale bars: A-D, E, and F, 40 um; D' and F', 10 um; and G, 50 um.

Copyright

This image is from Peng YJ, J Biol Chem 2010 Aug 6;285(32):24834-44 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165902

Associated Alleles

Symbol	Name
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein
Trio^tm1Mzhu	triple functional domain (PTPRF interacting); targeted mutation 1, Min-Sheng Zhu

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Nes-cre)1Kln/0 Trio^tm1Mzhu/Trio^tm1Mzhu	involves: 129S6/SvEvTac * C57BL/6 * SJL