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Gene Expression Data
Assay Details
Assay
Reference: J:254842 Fujita J, et al., Ronin Governs Early Heart Development by Controlling Core Gene Expression Programs. Cell Rep. 2017 Nov 7;21(6):1562-1573
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6714260
Gene symbol: Actn2
Gene name: actinin alpha 2
Antibody: anti alpha-actinin (EA-53)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S3D Heterozygous control: embryonic day 11.5; Thap11tm1Tpz/Thap11+, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant. Double labeled: green - Actn2; red - phospho-Histone 3.
Structure Level Pattern Image Note
TS19: heart Present S3D top left , S3D bottom left

Specimen S3D Ronin knockout: embryonic day 11.5; Thap11tm1Tpz/Thap11tm1Tpz, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant. Double labeled: green - Actn2; red - phospho-Histone 3.
Structure Level Pattern Image Note
TS19: heart Present S3D bottom right , S3D top right

Specimen S4A: embryonic day 13.5; Gt(ROSA)26Sortm1Hjf/Gt(ROSA)26Sortm1Hjf, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Actn2; red - Rosa26-RFP.
Structure Level Pattern Image Note
TS21: cardiac muscle tissue Present S4A Merge , S4A alpha-Actinin

Specimen S9B Control: embryonic day 11.5; Thap11tm1Tpz/Thap11+, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant. Double labeled: green - Actn2; red - Rps6 (assay MGI:6714277).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Present S9B Control alpha-Actinin , S9B Control Merge

Specimen S9B Ronin KO: embryonic day 11.5; Thap11tm1Tpz/Thap11tm1Tpz, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant. Double labeled: green - Actn2; red - Rps6 (assay MGI:6714277).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Present S9B Ronin KO alpha-Actinin , S9B Ronin KO Merge Actn2 expression level was not affected in the knockout, while Rps6 expression was lower.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/12/2021
MGI 6.17
The Jackson Laboratory