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Gene Expression Data
Assay Details
Assay
Reference: J:148786 Barber M, et al., The role of Robo3 in the development of cortical interneurons. Cereb Cortex. 2009 Jul;19 Suppl 1:i22-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5752745
Gene symbol: Robo1
Gene name: roundabout guidance receptor 1
Antibody: anti-Robo1
Detection system: Biotinylated secondary antibody/[Strept]avidin
Results
Specimen 2A/A': embryonic day 13.5 (more )
Note: Double labeled: green - Robo1; red - Reln (assay MGI:5752750).
Structure Level Pattern Image Note
TS21: cerebral cortex marginal layer Present Regionally restricted 2A , 2A' Expressed within the lower half of the preplate/marginal zone and below a single layer of strongly labeled reelin immunopositive cells, a marker of Cajal--Retzius cells.
TS21: cortical preplate Present Regionally restricted 2A , 2A' Expressed within the lower half of the preplate/marginal zone and below a single layer of strongly labeled reelin immunopositive cells, a marker of Cajal--Retzius cells.
TS21: cerebral cortex Present Regionally restricted 2A , 2A' Colocalization with reelin was also more prominent within the deeper layers of the cortical hem.

Specimen S1A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted S1A Expressed in the preplate layer, the lateral, medial and caudal ganglionic eminences.

Specimen S1D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cortical preplate Present Graded S1D In a decreasing mediolateral gradient.
TS20: lateral ganglionic eminence Present Regionally restricted S1D Robo1 and Robo3 showed similar patterns of expression both within the lateral and medial ganglionic eminences, and overlapped in regions where Robo1 protein was most robustly localized.
TS20: medial ganglionic eminence Present Regionally restricted S1D Robo1 and Robo3 showed similar patterns of expression both within the lateral and medial ganglionic eminences, and overlapped in regions where Robo1 protein was most robustly localized.

Specimen S1G: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Strong Regionally restricted S1G Expressed within the cortical hem, and this extended in a decreasing mediolateral gradient throughout the preplate/marginal zone.
TS21: cerebral cortex marginal layer Strong Graded S1G Expressed in a decreasing mediolateral gradient throughout the preplate/marginal zone.
TS21: cortical preplate Strong Graded S1G Expressed in a decreasing mediolateral gradient throughout the preplate/marginal zone.

Specimen not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: caudal ganglionic eminence Strong


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory