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Gene Expression Data
Assay Details
Assay
Reference: J:176654 McCormick JA, et al., A SPAK Isoform Switch Modulates Renal Salt Transport and Blood Pressure. Cell Metab. 2011 Sep 7;14(3):352-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5471843
Gene symbol: Stk39
Gene name: serine/threonine kinase 39
Antibody: N-SPAK antibody
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2A top, merge: postnatal (more )
Note: Double staining with NCC as hown in assay MGI:5472011.
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 2A N-SPAK top , 2A top merge
TS28: loop of Henle distal straight tubule Present 2A N-SPAK top , 2A top merge

Specimen 2A bottom, merge: postnatal (more )
Note: Double staining with NKCC2 as hown in assay MGI:5472012.
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 2A bottom merge , 2A N-SPAK bottom
TS28: loop of Henle distal straight tubule Present 2A bottom merge , 2A N-SPAK bottom

Specimen 2B top, merge: postnatal (more )
Note: Double staining with NCC as hown in assay MGI:5472011.
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 2B N-SPAK top , 2A top merge
TS28: loop of Henle distal straight tubule Present 2B N-SPAK top , 2A top merge

Specimen 3B Wild Type: postnatal (more )
Structure Level Pattern Image Note
TS28: renal cortex Present 3B Wild Type
TS28: renal medulla Present 3B Wild Type

Specimen 3B HET: postnatal; Stk39tm1Dlp/Stk39+ (more )
Structure Level Pattern Image Note
TS28: renal cortex Present 3B HET
TS28: renal medulla Present 3B HET

Specimen 3B Knockout: postnatal; Stk39tm1Dlp/Stk39tm1Dlp (more )
Structure Level Pattern Image Note
TS28: renal cortex Absent 3B Knockout
TS28: renal medulla Absent 3B Knockout


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/05/2021
MGI 6.17
The Jackson Laboratory