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Gene Expression Data
Assay Details
Assay
Reference: J:176654 McCormick JA, et al., A SPAK Isoform Switch Modulates Renal Salt Transport and Blood Pressure. Cell Metab. 2011 Sep 7;14(3):352-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5472011
Gene symbol: Slc12a3
Gene name: solute carrier family 12, member 3
Antibody: NCC antibody
Detection system: Secondary antibody
Results
Specimen 2A top, merge: postnatal (more )
Note: Double labeled: red - Slc12a3 (NCC); green - Stk39 (SPAK; assay MGI:5471843).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 2A NCC , 2A top merge
TS28: loop of Henle distal straight tubule Present 2A NCC , 2A top merge
Specimen 2B top, merge: postnatal (more )
Note: Double labeled: red - Slc12a3 (NCC); green - Stk39 (SPAK; assay MGI:5471843).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 2B NCC top , 2B top merge
TS28: loop of Henle distal straight tubule Present 2B NCC top , 2B top merge
Specimen 2B bottom, merge: postnatal (more )
Note: Double labeled: red - Slc12a3 (NCC); green - Stk39 (SPAK; assay MGI:5472010).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 2B NCC bottom , 2B bottom merge
TS28: loop of Henle distal straight tubule Present 2B NCC bottom , 2B bottom merge
Specimen 5A Wild Type: postnatal (more )
Note: Double labeled: green - Slc12a3 (total protein; this assay); red - Slc12a3 (pT53-NCC; assay MGI:5473386).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5A Wild Type NCC , 5A Wild Type Merge
Specimen 5A Knockout: postnatal; Stk39tm1Dlp/Stk39tm1Dlp (more )
Note: Double labeled: green - Slc12a3 (total protein; this assay); red - Slc12a3 (pT53-NCC; assay MGI:5473386).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5A Knockout Merge , 5A Knockout NCC
Specimen 5B Wild Type: postnatal (more )
Note: Double labeled: green - Slc12a3 (total protein; this assay); red - Slc12a3 (pS71-NCC; assay MGI:5473383).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5B Wild Type NCC , 5B Wild Type Merge
Specimen 5B Knockout: postnatal; Stk39tm1Dlp/Stk39tm1Dlp (more )
Note: Double labeled: green - Slc12a3 (total protein; this assay); red - Slc12a3 (pS71-NCC; assay MGI:5473383).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5B Knockout Merge , 5B Knockout NCC
Specimen 5E Wild Type: postnatal (more )
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5E Wild Type
Specimen 5E Knockout: postnatal; Stk39tm1Dlp/Stk39tm1Dlp (more )
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5E Knockout Expression is present, the mutant has less distal convoluted tubules segments.
Specimen 6B Wild Type, merge: postnatal (more )
Note: Double labeled: red - Slc12a3 (NCC); green - Oxsr1 (assay MGI:5472074).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 6B top merge , 6B NCC Wild-Type
Specimen 6B KO, merge: postnatal; Stk39tm1Dlp/Stk39tm1Dlp (more )
Note: Double labeled: red - Slc12a3 (NCC); green - Oxsr1 (assay MGI:5472074).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 6B NCC KO , 6B bottom merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory