Antibody:	anti-sialin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Very strong
TS28: hippocampus pyramidal cell layer	Very strong
TS28: hippocampus stratum oriens	Very strong
TS28: hippocampus stratum radiatum	Very strong
TS28: red nucleus	Very strong
TS28: accumbens nucleus	Strong
TS28: cerebellum Purkinje cell layer	Strong
TS28: cortical layer I	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cortical layer IV	Strong
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong
TS28: dentate gyrus granule cell layer	Strong
TS28: dentate gyrus molecular layer	Strong
TS28: globus pallidus	Strong
TS28: interpeduncular nucleus	Strong
TS28: lateral septal nucleus	Strong
TS28: reticular tegmental nucleus	Strong
TS28: substantia nigra pars compacta	Strong
TS28: amygdalopiriform transition area	Moderate
TS28: arcuate nucleus	Moderate
TS28: central thalamic nucleus	Moderate
TS28: claustrum	Moderate
TS28: dentate gyrus polymorphic layer	Moderate
TS28: endopiriform nucleus	Moderate
TS28: hippocampus stratum lacunosum	Moderate
TS28: islands of Calleja	Moderate
TS28: locus coeruleus	Moderate
TS28: medial septal nucleus	Moderate
TS28: nucleus of trapezoid body	Moderate
TS28: nucleus reuniens	Moderate
TS28: olfactory tubercle	Moderate
TS28: paraventricular hypothalamic nucleus	Moderate
TS28: periaqueductal grey	Moderate
TS28: piriform cortex	Moderate
TS28: pontine nucleus	Moderate
TS28: raphe magnus nucleus	Moderate
TS28: subiculum	Moderate
TS28: suprachiasmatic nucleus	Moderate
TS28: ventromedial hypothalamic nucleus	Moderate
TS28: cerebellum molecular layer	Weak
TS28: dorsomedial hypothalamic nucleus	Weak
TS28: hypothalamus periventricular zone	Weak
TS28: inferior colliculus	Weak
TS28: lateral habenular nucleus	Weak
TS28: lateral hypothalamic area	Weak
TS28: medial habenular nucleus	Weak
TS28: nucleus of diagonal band	Weak
TS28: pallidum	Weak
TS28: paracentral nucleus	Weak
TS28: paraventricular thalamic nucleus	Weak
TS28: raphe pallidus nucleus	Weak
TS28: reticular thalamic nucleus	Weak
TS28: substantia nigra pars reticulata	Weak
TS28: superior colliculus	Weak
TS28: superior olivary nucleus	Weak
TS28: trigeminal nucleus	Weak
TS28: forebrain	Present	Regionally restricted		Expression is strong in the indusium griseum and weak in the subtstantia innominata.
TS28: basolateral amygdaloid nucleus	Strong			Moderate to high expression.
TS28: basomedial amygdaloid nucleus	Strong			Moderate to high expression.
TS28: medial amygdaloid nucleus	Strong			Moderate to high expression.
TS28: posterolateral cortical amygdaloid nucleus	Strong			Moderate to high expression.
TS28: posteromedial cortical amygdaloid nucleus	Strong			Moderate to high expression.
TS28: amygdala	Strong	Regionally restricted		Moderate to high expression in the central amyddaloid nucleus.
TS28: anterior cortical amygdaloid nucleus	Moderate			Weak to moderate expression.
TS28: lateral amygdaloid nucleus	Moderate			Weak to moderate expression.
TS28: midbrain tegmentum	Weak	Regionally restricted		Expression is ventral.
TS28: midbrain	Present	Regionally restricted		Expression is moderate in the lateral lemniscus nucleus, weak in the deep mesencephalic nuclei and is trace in the mesencephalic trigeminal nucleus.
TS28: raphe nucleus	Present	Regionally restricted		Expression is moderate in the median raphe nucleus and is weak in the dorsal-, paramedian nucleus , and in the linear nucleus of raphe.
TS28: cerebellum granule cell layer	Weak			Weak to trace expression.
TS28: corpus callosum	Weak			Weak to trace expression.
TS28: fimbria hippocampus	Weak			Weak to trace expression.
TS28: cochlear nucleus	Moderate	Regionally restricted		Expression is in the ventral cochlear nucleus.
TS28: cerebral hemisphere white matter	Trace	Regionally restricted		Absent in the the external and internal capsule (weak to trace in corpus callosum and fimbria of hippocampus).
TS28: hypothalamus	Weak	Regionally restricted		Expression is in the anterior hypothalamic area.
TS28: thalamus	Weak	Regionally restricted		Expression is in the anterodorsal and anteromedial thalamic nuclei, the interarterodorsal and interarteromedial thalmice nuclei, and the ventral anterior and ventrolateral thalamic nuclei.
TS28: lateral thalamic group	Weak	Regionally restricted		Expression is in the laterodorsal thalamic nuclei.
TS28: anterior commissure	Absent
TS28: cerebellum white matter	Absent			Absent in the arbor vitae.

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Strong		2A
TS28: cerebral cortex	Strong		2A
TS28: corpus striatum	Strong		2A

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Strong		2B
TS28: cerebral cortex	Strong		2B
TS28: corpus striatum	Strong		2B
TS28: hippocampus	Strong		2B

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong		2D	Immunoreactivity was observed mainly in the neuropil.
TS28: cortical layer II	Strong		2D	Immunoreactivity was observed mainly in the neuropil.
TS28: cortical layer III	Strong		2D	Immunoreactivity was observed mainly in the neuropil.
TS28: cortical layer IV	Strong		2D	Immunoreactivity was observed mainly in the neuropil.
TS28: cortical layer V	Strong		2D	Immunoreactivity was observed mainly in the neuropil.
TS28: cortical layer VI	Strong		2D	Immunoreactivity was observed mainly in the neuropil.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Very strong		2E	Large pyramidal neurons having high cytoplasmic immunoreactivity.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Very strong		2F	Immunoreactivity was observed mainly in the neuropil.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong		2G	Expression is in the perikarya of the pyramidal cells within the CA2 and CA3 (shown) areas of the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: globus pallidus	Present		2H	Expression is in the lateral globus pallidus.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		2I

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present			Low to moderate expression.
TS28: enteric nervous system	Present	Regionally restricted		Expression is in the enteric nervous system neurons of the gut.
TS28: gut	Weak	Regionally restricted		Expression is in epithelial cells.
TS28: spleen	Absent
TS28: thymus	Absent

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present		3A	Expression is in the anterodorsal thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS28: reticular thalamic nucleus	Present		3B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3C
TS28: cerebellum molecular layer	Weak		3C
TS28: cerebellum granule cell layer	Absent		3C

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Present		3D

Structure	Level	Pattern	Image	Note
TS28: red nucleus	Present	Regionally restricted	3E	Expression is in the magnocellular part.

Structure	Level	Pattern	Image	Note
TS28: fimbria hippocampus	Weak	Single cells	3F	Immunoreactivity was observed in some glial cells within the fimbria of the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: raphe nucleus	Present	Regionally restricted	3G	Expression is in the median raphe nucleus.

Structure	Level	Pattern	Image	Note
TS28: nucleus of trapezoid body	Present		3H
TS28: raphe magnus nucleus	Present		3H

Structure	Level	Pattern	Image	Note
TS28: nucleus of trapezoid body	Present		3I

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Present		3J

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present		3K

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Weak	Diffuse	4A

Structure	Level	Pattern	Image	Note
TS28: renal distal tubule	Present	Regionally restricted	4B	Expression is in the apical parts.
TS28: renal proximal tubule	Weak	Regionally restricted	4B	Expression is in the epithelium.
TS28: glomerular tuft	Weak		4B

Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Present		4C

Structure	Level	Pattern	Image	Note
TS28: brain	Present		4D

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	4E	Expression is in a small population.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Weak	Regionally restricted	4F	Expression is in some endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		4G	Expression was seen in cells at different stages of spermatogenesis in the testes

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		4H	The ganglion cell labeling intensity was higher in the larger neurons, with some of those exhibiting very strong immunoreactivity both in the cytoplasm and on the plasma membrane.

Structure	Level	Pattern	Image	Note
TS19: brain	Moderate	Regionally restricted	5A	Low to moderate (marginal layer) expression.
TS19: head	Present	Regionally restricted	5A	Low to moderate staining was present in the developing brain and mesenchyme, with no immunoreactivity in other tissues.
TS19: head mesenchyme	Moderate		5A
TS19: brain ventricular layer	Absent		5A

Structure	Level	Pattern	Image	Note
TS23: brain	Strong	Regionally restricted	5B
TS23: cerebral cortex ventricular layer	Weak		5B
TS23: forebrain	Weak		5B
TS23: midbrain	Weak	Regionally restricted	5B	Expression is in the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5C	Expression is in the precortical plate.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	5D	Expression is in the precortical plate.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		5E
TS23: cerebral cortex marginal layer	Present		5E
TS23: cortical subplate	Present		5E

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		5F
TS26: cerebral cortex marginal zone	Present		5F
TS26: cerebral cortex subventricular zone	Absent		5F
TS26: cerebral cortex ventricular zone	Absent		5F
TS26: cortical plate	Absent		5F
TS26: cortical subplate	Absent		5F

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong		5H

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong		5I

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Moderate		6A	Low to moderate levels.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Moderate		6B
TS19: facial ganglion	Moderate		6B

Structure	Level	Pattern	Image	Note
TS19: glossopharyngeal ganglion	Moderate	Regionally restricted	6C	Expression is in extending axons.
TS19: superior vagus ganglion	Moderate	Regionally restricted	6C	Expression is in extending axons.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Moderate	Regionally restricted	6D	Expression is in extending axons.

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Moderate		6E
TS19: neural tube marginal layer	Moderate		6E

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		7A

Structure	Level	Pattern	Image	Note
TS21: vestibulo-cochlear ganglion	Present		7B

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong		7C

Structure	Level	Pattern	Image	Note
TS23: central nervous system ganglion	Strong	Regionally restricted	8A	Expression is in the heart ganglion.

Structure	Level	Pattern	Image	Note
TS25: parasympathetic nervous system	Strong	Regionally restricted	8B	Expression is in the submandibular gland parasympathetic ganglion.

Structure	Level	Pattern	Image	Note
TS25: cochlear ganglion	Strong		8C

Structure	Level	Pattern	Image	Note
TS23: olfactory nerve	Strong		8D

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Strong		8E

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Strong		8F

Structure	Level	Pattern	Image	Note
TS25: inferior cervical ganglion	Strong		8G

Structure	Level	Pattern	Image	Note
TS25: thoracic ganglion	Strong		8H

Structure	Level	Pattern	Image	Note
TS23: parasympathetic nervous system	Strong		8I	Expression is in intra-lingual parasympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS21: midgut	Present	Regionally restricted	9A	Expression is in neural crest-derived cells residing in the outer mesenchyme of the midgut.

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Present	Regionally restricted	9B	Expression is in the myenteric plexi.
TS23: midgut	Present	Regionally restricted	9B	Expression is in the myenteric plexi.

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Present	Regionally restricted	9C	Expression is in the myenteric plexi.
TS23: esophagus	Present	Regionally restricted	9C	Expression is in the myenteric plexi.

Structure	Level	Pattern	Image	Note
TS24: myenteric nerve plexus	Present	Regionally restricted	9D	Expression is in the myenteric plexi.
TS24: stomach	Present	Regionally restricted	9D	Expression is in the myenteric plexi.

Structure	Level	Pattern	Image	Note
TS24: gut	Present	Regionally restricted	9E	Expression is in the myenteric plexi of the midgut.
TS24: myenteric nerve plexus	Present	Regionally restricted	9E	Expression is in the myenteric plexi of the midgut.

Structure	Level	Pattern	Image	Note
TS25: myenteric nerve plexus	Present	Regionally restricted	9F	Expression is in the myenteric plexi.
TS25: rectum	Present	Regionally restricted	9F	Expression is in the myenteric plexi.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present
TS19: peripheral nervous system	Present
TS19: embryo	Weak	Non-Uniform		Expression is mainly in the central and peripheral nervous system, with much lower staining intensity in other tissues and organs.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present
TS21: peripheral nervous system	Present
TS21: embryo	Weak	Non-Uniform		Expression is mainly in the central and peripheral nervous system, with much lower staining intensity in other tissues and organs.
TS21: forebrain	Strong	Regionally restricted		Expression is in the basal forebrain and hippocampus.
TS21: hypothalamus	Strong
TS21: midbrain	Strong
TS21: pons	Strong
TS21: thalamus	Strong
TS21: brain	Present	Regionally restricted		Expression is absent in the ventricular and subventricular zones.
TS21: cardiac muscle tissue	Weak	Diffuse

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present
TS23: peripheral nervous system	Present
TS23: embryo	Weak	Non-Uniform		Expression is mainly in the central and peripheral nervous system, with much lower staining intensity in other tissues and organs.
TS23: cardiac muscle tissue	Weak	Diffuse
TS23: lung	Weak	Regionally restricted		Expression is in a small population.
TS23: liver	Weak	Regionally restricted		Expression is in hepatocytes and megakaryocytes.
TS23: pancreas	Weak	Regionally restricted		Expression is in the islands of Langerhans.
TS23: metanephros	Present	Regionally restricted		Moderate expression in in distal tubules, with low staining in the glomeruli.

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present
TS24: peripheral nervous system	Present
TS24: embryo	Weak	Non-Uniform		Expression is mainly in the central and peripheral nervous system, with much lower staining intensity in other tissues and organs.
TS24: cardiac muscle tissue	Weak	Diffuse
TS24: lung	Weak	Regionally restricted		Expression is in a small population.
TS24: liver	Weak	Regionally restricted		Expression is in hepatocytes and megakaryocytes.
TS24: pancreas	Weak	Regionally restricted		Expression is in the islands of Langerhans.
TS24: metanephros	Present	Regionally restricted		Moderate expression in in distal tubules, with low staining in the glomeruli.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present
TS25: peripheral nervous system	Present
TS25: embryo	Weak	Non-Uniform		Expression is mainly in the central and peripheral nervous system, with much lower staining intensity in other tissues and organs.
TS25: cardiac muscle tissue	Weak	Diffuse
TS25: lung	Weak	Regionally restricted		Expression is in a small population.
TS25: liver	Weak	Regionally restricted		Expression is in hepatocytes and megakaryocytes.
TS25: pancreas	Weak	Regionally restricted		Expression is in the islands of Langerhans.
TS25: renal distal tubule	Moderate
TS25: glomerular tuft	Weak

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present
TS26: peripheral nervous system	Present
TS26: embryo	Weak	Non-Uniform		Expression is mainly in the central and peripheral nervous system, with much lower staining intensity in other tissues and organs.