Phenotypes Associated with This Genotype

Genotype
MGI:8407904

• Allelic Composition: Mtfr1l^em2Gpt/Mtfr1l^em2Gpt; Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl}/Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl}
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal mitophagy ( J:390197 )

• 2-month-old mice exhibit impaired cardiac mitophagy in response to exhaustive exercise, showing a significantly lower count of mitolysosomes (pure red puncta per 1000 um²) in heart tissue at 1 h postexercise than control mice homozygous for Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl} alone

• in response to exhaustive exercise, accumulation of phosphorylated Ubiquitin at Serine 65 (p-S65-Ub) and degradation of PRKN (parkin RBR E3 ubiquitin protein ligase; aka Parkin) are significantly blunted, suggesting impaired signal propagation through the PINK1/ Parkin axis

• however, basal mitophagy levels are not significantly altered under resting conditions and levels of mitochondrial dynamics-related proteins (MFN2, DRP1 and OPA1) remain unchanged during exhaustive exercise

increased circulating interleukin-6 level ( J:390197 )

• at 1 h postexercise, 2-month-old mice exhibit a significant increase in plasma IL-6 levels

• however, baseline IL-6 levels are similar to those in control mice

abnormal response to exercise ( J:390197 )

• 2-month-old mice exhibit impaired cardiac mitophagy in response to exhaustive exercise

cardiovascular system

abnormal myocardial fiber mitochondrial morphology ( J:390197 )

• at 1 h postexercise, 2-month-old mice exhibit a significant reduction in healthy mitochondria along with an increase in mildly damaged mitochondria in heart tissue relative to control mice based on morphological criteria

cellular

abnormal myocardial fiber mitochondrial morphology ( J:390197 )

• at 1 h postexercise, 2-month-old mice exhibit a significant reduction in healthy mitochondria along with an increase in mildly damaged mitochondria in heart tissue relative to control mice based on morphological criteria

abnormal mitophagy ( J:390197 )

• 2-month-old mice exhibit impaired cardiac mitophagy in response to exhaustive exercise, showing a significantly lower count of mitolysosomes (pure red puncta per 1000 um²) in heart tissue at 1 h postexercise than control mice homozygous for Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl} alone

• in response to exhaustive exercise, accumulation of phosphorylated Ubiquitin at Serine 65 (p-S65-Ub) and degradation of PRKN (parkin RBR E3 ubiquitin protein ligase; aka Parkin) are significantly blunted, suggesting impaired signal propagation through the PINK1/ Parkin axis

• however, basal mitophagy levels are not significantly altered under resting conditions and levels of mitochondrial dynamics-related proteins (MFN2, DRP1 and OPA1) remain unchanged during exhaustive exercise

immune system

increased circulating interleukin-6 level ( J:390197 )

• at 1 h postexercise, 2-month-old mice exhibit a significant increase in plasma IL-6 levels

• however, baseline IL-6 levels are similar to those in control mice

muscle

abnormal myocardial fiber mitochondrial morphology ( J:390197 )

• at 1 h postexercise, 2-month-old mice exhibit a significant reduction in healthy mitochondria along with an increase in mildly damaged mitochondria in heart tissue relative to control mice based on morphological criteria