Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ian Ganley
• MGI ID: MGI:5817670
• Synonyms: mito-QC
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mCherry/GFP)Ganl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237975
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector consists of (from 5' to 3') an FRT3 site, PGK neo polyA cassette, a CAG promoter, a Kozak sequence (GCCACC), the mCherry-GFP-mtFIS1<101-152> coding sequence (details below), a human growth hormone polyA sequence (hGHpA), an additional polyA sequence and a FRT site. Recombination mediated cassette exchange (RMCE) is used to insert the CAG promoter and the open reading frame for the mCherry-GFP-mtFIS1<101-152> fusion protein into the Gt(ROSA)26Sor locus. The mCherry-GFP-mtFIS1<101-152> fusion protein (mito-QC) is a cDNA sequence composed of (from 5' to 3') a monomeric red fluorescent protein (mCherry) fused in-frame to a green fluorescent protein (GFP), then an in-frame outer mitochondrial membrane targeting sequence (amino acids 101-152 from the human FIS1 locus [fission, mitochondrial 1]). Under steady-state conditions, the mitochondrial network exhibits both mCherry (red) fluorescence and GFP (green) fluorescence. Upon mitophagy, mitochondria are delivered to lysosomes where mCherry fluorescence remains stable, but GFP fluorescence becomes quenched by the acidic (low pH) microenvironment. (J:237975)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:237975 McWilliams TG, et al., mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol. 2016 Aug 01;214(3):333-45
• All: 10 reference(s)