Phenotypes Associated with This Genotype

Genotype
MGI:6406415

• Allelic Composition: Chn1^tm1.2Ece/Chn1^tm1.2Ece; Epha4^tm1.2Bzh/Epha4^tm1.2Bzh; Tg(Hlxb9-GFP)1Tmj/0
• Genetic Background: involves: 129S/Sv * C57BL/6J * CD-1 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal innervation ( J:243785 )

• however, embryos show normalized abducens exit and fasciculation

• 2 of 5 embryos show orbital innervation failure by abducens nerve

• abducens nerve shows enhanced wandering of abducens bundles

• embryos show increased abducens nerve stalling compared to homozygous Chn1^tm1.1Ece homozygotes and similar abducens nerve wandering compared to Epha4^tm1.2Bzh homozygotes

• stalled projections do not stay within normal nerve boundaries and instead wander to track with the mandibular and cervical branches of the facial nerve

• the abducens nerve fails to innervate the lateral rectus at E16.5, which instead is innervated by aberrant branches of the oculomotor nerve; this phenotype is worse than seen in single Epha4^tm1.2Bzh homozygotes

abnormal abducens nerve morphology ( J:243785 )

• embryos show a reduction in abducens nerve length and diameter at the orbit