Tg(Hlxb9-GFP)1Tmj
Transgene Detail

Summary

• Symbol: Tg(Hlxb9-GFP)1Tmj
• Name: transgene insertion 1, Thomas M Jessell
• MGI ID: MGI:3056906
• Synonyms: Gfp-HB9, Hb9-eGFP, Hb9::EGFP, Hb9-Gfp, Hb9:GFP-1B, Hlxb9:GFP
• Transgene: Tg(Hlxb9-GFP)1Tmj Location: Chr15:13853202-13862183 bp Genetic Position: Chr15, Syntenic
• Alliance: Tg(Hlxb9-GFP)1Tmj page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 (Mnx1) gene, a 5' splice substrate, an enhanced Green Fluorescent Protein (EGFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. Line 1 contains 10 to 11 tandem copies of the transgene. The expression pattern of this transgene is the same as that for the endogenous gene from which the promoter is derived. (J:88764, J:271391)

Disease models

Expression

In Mice Carrying this Mutation:

4 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

Notes

Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice.

References

• Original: J:88764 Wichterle H, et al., Directed differentiation of embryonic stem cells into motor neurons. Cell. 2002 Aug 9;110(3):385-97
• All: 129 reference(s)