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Phenotypes Associated with This Genotype
Genotype
MGI:5696659
Allelic
Composition
Nmt1tm1.1Poru/Nmt1tm1.1Poru
Nmt2tm1.1Poru/Nmt2tm1.1Poru
Tg(Lck-cre)1Cwi/0
Genetic
Background
involves: C57BL/6 * DBA/2 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nmt1tm1.1Poru mutation (0 available); any Nmt1 mutation (54 available)
Nmt2tm1.1Poru mutation (0 available); any Nmt2 mutation (6 available)
Tg(Lck-cre)1Cwi mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• mice do not exhibit any signs of autoimmune disease
• during all stages of T cell development except DN1 that does not express the cre transgene
• 10-fold in double positive cells
• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice
• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice
• when stimulated with IL2
• however, cortical area is normal
• in the thymus more so than single conditional knock-outs
• in the blood, lymph nodes and spleen as in Nmt1tm1.1Poru conditional knock-outs
• at 2 and 6 months more so than in either single conditional knock-out
• CD4+ and CD8+ single positive cells at 2 and 6 months
• following treatment with CpC-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice
• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes
• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes
• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B
• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B
• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B
• after TCR-independent activation
• after TCR-independent activation
• mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B exhibit reduced plasma levels of TNF, IL2 and IFN-gamma compared with wild-type mice

homeostasis/metabolism
• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B
• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B
• in mice sensitized with D-galactosamine hydrochloride and treated with staphylococcal enterotoxin B

cellular
• during all stages of T cell development except DN1 that does not express the cre transgene
• 10-fold in double positive cells
• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice
• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice
• when stimulated with IL2

endocrine/exocrine glands
• however, cortical area is normal

hematopoietic system
• during all stages of T cell development except DN1 that does not express the cre transgene
• 10-fold in double positive cells
• following treatment with CpG-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice
• however, untreated mice exhibit normal T cell proliferation and treated mice do exhibit an increase in macrophages in the spleen and liver as in wild-type mice
• when stimulated with IL2
• however, cortical area is normal
• in the thymus more so than single conditional knock-outs
• in the blood, lymph nodes and spleen as in Nmt1tm1.1Poru conditional knock-outs
• at 2 and 6 months more so than in either single conditional knock-out
• CD4+ and CD8+ single positive cells at 2 and 6 months
• following treatment with CpC-ODNs, mice fail to exhibit an increased in CD4+ and CD8+ single positive T cells compared with wild-type mice
• CD4+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes
• CD8+ T cells isolated from the lymph nodes and spleen exhibit a shift from naive to activated/memory phenotypes


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last database update
12/04/2018
MGI 6.13
The Jackson Laboratory