Phenotypes Associated with This Genotype

Genotype
MGI:5588382

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Grem1)Svok}/Gt(ROSA)26Sor⁺; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: 129S1/Sv * C57BL/6J * SJL/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

limbs/digits/tail

abnormal forelimb bud morphology ( J:214075 )

• forelimbs of most (7/10) E11.5 embryos have shriveled limb buds with small patches of apical ectodermal ridge (AER) as compared to controls

abnormal digit morphology ( J:214075 )

• hindlimb phalanges have delays in ossification that is most obvious at tips

• hindlimb digits (E18.5) are more uniform in length as compared to controls

abnormal hallux morphology ( J:214075 )

• all embryos exhibit a transformation of digit 1 from a shorter digit with 2 phalanges to a longer digit with 3 phalanges

brachydactyly ( J:214075 )

• hindlimb digits (E18.5) are shorter than controls

• shortened digits are reduced proportionally

forelimb oligodactyly ( J:214075 )

• 2 embryos (2/10) have reduced numbers of digits on both forelimbs

polydactyly ( J:214075 )

• 1 embryo (1/10) has polydactyly on both forelimbs

• hindlimb autopods are polydactyl (6-9 digits) with soft tissue syndactyly

syndactyly ( J:214075 )

• hindlimbs are polydactyl (6-9 digits) with soft tissue syndactyly

triphalangia ( J:214075 )

absent forelimb ( J:214075 )

• most E11.5 embryos (7/10) lack forelimbs and have only residual cartilage attached to the scapula

abnormal hindlimb morphology ( J:214075 )

• size of hindlimbs is visibly increased by E11.5

embryo

abnormal forelimb bud morphology ( J:214075 )

• forelimbs of most (7/10) E11.5 embryos have shriveled limb buds with small patches of apical ectodermal ridge (AER) as compared to controls

cellular

decreased apoptosis ( J:214075 )

• hindlimbs have reduced levels of apoptosis in anterior AER and mesoderm beginning at E11.75

• numbers of apoptotic cells in the posterior zone are reduced in limb buds

• normal anterior necrotic zone is absent

increased cell proliferation ( J:214075 )

• hindlimbs have a 20% increase in mitotic index as compared to controls