mortality/aging
• mice become moribund at 30 days of age and are euthanized
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growth/size/body
• although normal at birth, mice become growth retarded after 14 days of age
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homeostasis/metabolism
• glutathione levels in the liver are about 37% of normal at 14 days of age
• glutathione levels in plasma are extremely low at 14 days of age
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• plasma levels are dramatically elevated by 28 days of age
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• plasma levels are dramatically elevated by 28 days of age
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liver/biliary system
• vacuolated hepatocytes are diffusely present at 21 days of age
• both macrovesicular and microvesicular vacuoles are found
• vacuoles contain neutral lipid
• scattered instances of single cell death
• increased number of binucleate hepatocytes
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• increasing steatosis from 21 days (26%) to 28 days of age (69%)
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cellular
• loss of lamellar rough endoplasmic reticulum in hepatocytes by 28 days of age
• replaced by small vesicles lacking ribosomes
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• 20% of mitochondria are morphologically abnormal in hepatocytes at 21 days of age
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• mitochondrion number in hepatocytes is 42% of control numbers at 14 days and 16% at 28 days of age
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• mitochondrial respiratory control ratios in hepatocytes are reduced at 21 days
• decreased oxygen consumption at state 3 respiration
• poorly coupled at 25 and 28 days
• decreased membrane potentials
• reduced liver ATP
• increased lipid peroxidation
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