Phenotypes Associated with This Genotype

Genotype
MGI:5317794

• Allelic Composition: Opa1^tm1.1Hise/Opa1^tm1.2Hise; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * DBA * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:183074 )

N • following intraperitoneal insulin injection, mice exhibit normal insulin tolerance

decreased insulin secretion ( J:183074 )

• in glucose-stimulated beta cells and mice

• however, unstimulated insulin secretion is normal

increased circulating glucose level ( J:183074 )

• under fasted and fed conditions

• in a glucose tolerance test

decreased circulating insulin level ( J:183074 )

• following intraperitoneal glucose injection

decreased oxygen consumption ( J:183074 )

• in islets in response to glucose stimulation

impaired glucose tolerance ( J:183074 )

endocrine/exocrine glands

abnormal pancreatic beta cell morphology ( J:183074 )

• decreased beta cell size and density

decreased pancreatic beta cell number ( J:183074 )

• in adult mice

small pancreatic islets ( J:183074 )

abnormal pancreatic beta cell physiology ( J:183074 )

• newborn mice exhibit decreased beta cell proliferation compared with control mice

• glucose-stimulated beta cells secrete less insulin compared with control cells

• beta cells exhibit impaired glucose-stimulated ATP production compared with control cells

• however, beta cells exhibit normal apoptosis and unstimulated insulin secretion

decreased pancreatic beta cell proliferation ( J:183074 )

• newborn mice exhibit decreased beta cell proliferation compared with control mice

decreased insulin secretion ( J:183074 )

• in glucose-stimulated beta cells and mice

• however, unstimulated insulin secretion is normal

cellular

abnormal mitochondrial morphology ( J:183074 )

• mitochondria in beta cells are highly fragmented, shorter with wider cristae compared to in control cells

• however, volume density of mitochondria is normal

abnormal mitochondrial crista morphology ( J:183074 )

• wider in beta cells compared with control cells

decreased pancreatic beta cell proliferation ( J:183074 )

• newborn mice exhibit decreased beta cell proliferation compared with control mice

abnormal respiratory electron transport chain ( J:183074 )

• islets exhibit decreased complex IV activity compared with control cells

• however, activity levels of complex I is normal