Phenotypes Associated with This Genotype

Genotype
MGI:4437914

• Allelic Composition: Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺; Plcg1^tm1Gcrp/Plcg1^tm1Rwen; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell apoptosis ( J:157757 )

• following reactivation

abnormal CD4-positive T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

abnormal CD8-positive, alpha-beta T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

increased double-positive T cell number ( J:157757 )

decreased T cell number ( J:157757 )

• peripheral T cells are reduced compared to in wild-type mice

• in transplantation experiments, T cells are less competitive than wild-type T cells

decreased CD4-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased single-positive T cell number ( J:157757 )

• CD4+ thymocytes are more profoundly decreased than CD8+ thymocytes

abnormal T cell activation ( J:157757 )

• mice exhibit an increase in T cell activation compared with wild-type mice

• however, activation phenotype is not cell intrinsic

decreased T cell proliferation ( J:157757 )

• in response to stimulation with anti-CD3m anti-CD3/anti-CD28, or anti-CD3/IL2, T cell proliferation is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

• however, treatment with PMA/ionomycin restores proliferation

abnormal regulatory T cell physiology ( J:157757 )

• YFP+ T regulatory cells exhibit reduced ability to suppress naive T cell proliferation compared with wild-type T cells

decreased interferon-gamma secretion ( J:157757 )

• following anti-CD3/anti-CD28 stimulation, slightly fewer IFN-gamma producing single positive T cells are detected compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

decreased interleukin-2 secretion ( J:157757 )

• following anti-CD3/anti-CD28 stimulation, IL2 production by single positive T cells is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

dermatitis ( J:157757 )

• mice exhibit dermatitis unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

growth/size/body

decreased body weight ( J:157757 )

• mice weight less than wild-type mice

• however, transfer of regulatory T cells restores normal weight gain

digestive/alimentary system

rectal prolapse ( J:157757 )

• mice exhibit rectal prolapse unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

hematopoietic system

increased T cell apoptosis ( J:157757 )

• following reactivation

abnormal CD4-positive T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

abnormal CD8-positive, alpha-beta T cell differentiation ( J:157757 )

• differentiation into CD4 or CD8 single positive thymocytes is reduced compared to in wild-type mice

increased double-positive T cell number ( J:157757 )

decreased T cell number ( J:157757 )

• peripheral T cells are reduced compared to in wild-type mice

• in transplantation experiments, T cells are less competitive than wild-type T cells

decreased CD4-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:157757 )

• in the thymus

decreased single-positive T cell number ( J:157757 )

• CD4+ thymocytes are more profoundly decreased than CD8+ thymocytes

abnormal T cell activation ( J:157757 )

• mice exhibit an increase in T cell activation compared with wild-type mice

• however, activation phenotype is not cell intrinsic

decreased T cell proliferation ( J:157757 )

• in response to stimulation with anti-CD3m anti-CD3/anti-CD28, or anti-CD3/IL2, T cell proliferation is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

• however, treatment with PMA/ionomycin restores proliferation

abnormal regulatory T cell physiology ( J:157757 )

• YFP+ T regulatory cells exhibit reduced ability to suppress naive T cell proliferation compared with wild-type T cells

integument

dermatitis ( J:157757 )

• mice exhibit dermatitis unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

alopecia ( J:157757 )

• mice exhibit alopecia unlike wild-type mice

• however, treatment with wild-type T regulatory cells prevents development of symptom

cellular

increased T cell apoptosis ( J:157757 )

• following reactivation

decreased T cell proliferation ( J:157757 )

• in response to stimulation with anti-CD3m anti-CD3/anti-CD28, or anti-CD3/IL2, T cell proliferation is reduced compared to in similarly treated Plcg1^tm1Gcrp/Plcg1⁺ Tg(CD4-cre)1Cwi Gt(ROSA)26Sor^tm1(EYFP)Cos mice

• however, treatment with PMA/ionomycin restores proliferation