Analysis Tools
mortality/aging
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• mice die between E11.5 and E15.5
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• mice die between E11.5 and E15.5
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limbs/digits/tail
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• authors state that mice exhibit limb defects observed in Hoxa13 knock-out homozygotes
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• cultured autopod mesenchyme cells exhibit reduced adhesion and form 8-fold fewer, smaller, and less robust precartilaginous aggregates compared with wild-type cells
• however, mesenchyme cells will contribute to large precartilaginous aggregates when co-cultured with wild-type cells by anchoring to wild-type cells
• autopod mesenchyme cells fail to segregate from wild-type cells as do cells from heterozygotes
• the autopod mesenchyme is disorganized and the boundaries between the mesoderm and epidermis is poorly defined unlike in wild-type mice
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syndactyly
(
J:72301
)
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• mice exhibit no interdigital separation between any of the fore limb or hind limb digits
• at E13.5, apoptosis between digits II and III is undetectable unlike in wild-type mice
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embryo
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• authors state that mice exhibit umbilical vascular defects observed in Hoxa13 knock-out homozygotes
• umbilical arteries exhibit a near complete loss of mesenchymal/endothelial cell layer stratification compared to in heterozygous mice
• the umbilical arteries exhibit disorganized vascular walls compared to in wild-type mice
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• at E10.5, umbilical arteries are narrower than in wild-type mice
• at E13.5, the lumen of umbilical vessels is ablated unlike in wild-type mice
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cardiovascular system
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• authors state that mice exhibit umbilical vascular defects observed in Hoxa13 knock-out homozygotes
• umbilical arteries exhibit a near complete loss of mesenchymal/endothelial cell layer stratification compared to in heterozygous mice
• the umbilical arteries exhibit disorganized vascular walls compared to in wild-type mice
|
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• at E10.5, umbilical arteries are narrower than in wild-type mice
• at E13.5, the lumen of umbilical vessels is ablated unlike in wild-type mice
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integument
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• thickened and distorted in autopods
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