Analysis Tools
mortality/aging
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• mice die by P21
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nervous system
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• at E14.5, fewer cells in the forebrain undergoing mitosis and more Tuj1+ and Oligo2+ cells undergoing apoptosis compared to in wild-type mice
• large numbers of cells in the forebrain at E14.5 and cerebellum at P7 undergo apoptosis unlike in wild-type mice
• however, no cell death is observed in astrocytes at P7 and E18.5
• in culture, neurospheres exhibit increased cell death comapre4d to wild-type cells
• in culture, neurospheres produce fewer Tuj1+ neurons and O4+oligodendrocytes compared to wild-type neurospheres
• in culture, neurosphere differentiation into GFAP+ astrocytes is abnormal
• cultured cerebellar granule cell precursors from P2 cerebellum exhibit impaired reaggregate formation and neurite projection compared to wild-type cells
• however, cultured neurospheres transfected with a constitutively active Prkab1 exhibit rescued self-renewal and cell survival
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• at E14.5, fewer cells in the forebrain undergoing mitosis and more Tuj1+ and Oligo2+ cells undergoing apoptosis compared to in wild-type mice
• at E18.5, 22% fewer mitotic cells around the lateral ventricles compared to in wild-type mice
• at P7, fewer cells in the dentate gyrus and cerebellum undergoing mitosis compared to in wild-type mice
• in culture, neurospheres are smaller than those that develop from wild-type cells
• proliferation of neuronal precursor cells is half of wild-type
• neuronal precursor cells exhibit defective mitosis in the G2M phase compared to wild-type cells
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• at E18.5 and P14, brain size is reduced by 50% compared to in wild-type
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• the dentate gyrus is nearly completely lost
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• mice exhibit between 35% and 65% loss in cortical and granule neurons in the dentate gyrus compared to in wild-type mice
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• reduction in the cerebral cortex results in improper cortical fusion and exposure of the superior and inferior colliculi unlike in wild-type mice
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• mice exhibit disordered laminar organization in the cerebellum unlike in wild-type mice
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• severe cerebellar atrophy
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• mice exhibit spongiform vacuolation
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• the number of astrocytes is increased compared to in wild-type mice
• at E18.5, the number of GFAP+ migrating astrocytes is greater than in wild-type mice
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astrocytosis
(
J:146618
)
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• extensive
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• at P14, mice exhibit a 75% to 80% loss in oligodendrocytes compared to in wild-type mice
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• mice exhibit loss of white matter axonal projections unlike in wild-type mice
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• mice exhibit widespread loss of dendritic processes unlike in wild-type mice
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• mice exhibit loss of neurons in the cortex, cerebellum, dentate gyrus, and hypothalamus compared to in wild-type mice
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• mice exhibit between 35% and 65% loss in cortical and granule neurons in the inner granule layer compared to in wild-type mice
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• translucent and hypomyelinated
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• 30% thinner than in wild-type mice
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• mice exhibit severe hypomyelination throughout the brain, particularly in the corpus callosum and striatum, compared to in wild-type mice
• the optic nerve is hypomyelinated
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behavior/neurological
growth/size/body
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• mice are emaciated at P14
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vision/eye
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• translucent and hypomyelinated
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• 30% thinner than in wild-type mice
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cellular
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• at E14.5, fewer cells in the forebrain undergoing mitosis and more Tuj1+ and Oligo2+ cells undergoing apoptosis compared to in wild-type mice
• large numbers of cells in the forebrain at E14.5 and cerebellum at P7 undergo apoptosis unlike in wild-type mice
• however, no cell death is observed in astrocytes at P7 and E18.5
• in culture, neurospheres exhibit increased cell death comapre4d to wild-type cells
• in culture, neurospheres produce fewer Tuj1+ neurons and O4+oligodendrocytes compared to wild-type neurospheres
• in culture, neurosphere differentiation into GFAP+ astrocytes is abnormal
• cultured cerebellar granule cell precursors from P2 cerebellum exhibit impaired reaggregate formation and neurite projection compared to wild-type cells
• however, cultured neurospheres transfected with a constitutively active Prkab1 exhibit rescued self-renewal and cell survival
|
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• at E14.5, fewer cells in the forebrain undergoing mitosis and more Tuj1+ and Oligo2+ cells undergoing apoptosis compared to in wild-type mice
• at E18.5, 22% fewer mitotic cells around the lateral ventricles compared to in wild-type mice
• at P7, fewer cells in the dentate gyrus and cerebellum undergoing mitosis compared to in wild-type mice
• in culture, neurospheres are smaller than those that develop from wild-type cells
• proliferation of neuronal precursor cells is half of wild-type
• neuronal precursor cells exhibit defective mitosis in the G2M phase compared to wild-type cells
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