Prkab1^{Gt(RRR454)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Prkab1^{Gt(RRR454)Byg}
• Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit; gene trap RRR454, BayGenomics
• MGI ID: MGI:3838355
• Synonyms: AMPKbeta1^-
• Gene: Prkab1 Location: Chr5:116151654-116162449 bp, - strand Genetic Position: Chr5, 56.1 cM, cytoband F
• Alliance: Prkab1^{Gt(RRR454)Byg} page

Mutation origin

• Mutant Cell Line: RRR454
• Germline Transmission: Earliest citation of germline transmission: J:146618
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector, which contains a beta-geo cassette, inserted downstream of exon 5. The fusion product lacks the terminal 46 animo acids required to form the active heterodimer. The absence of endogenous protein expression but expression of the lacZ fusion transcript was confirmed by western blot analysis on mouse embryonic fibroblasts and a variety of tissues, respectively. (J:146618)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR454	CG784753 ; (MGI Seq Detail)	5' RACE	Chr5:116156006-116158505 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkab1 Mutation: 24 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:146618 Dasgupta B, et al., AMP-activated protein kinase phosphorylates retinoblastoma protein to control mammalian brain development. Dev Cell. 2009 Feb;16(2):256-70
• All: 2 reference(s)