Phenotypes for Ar Plekha5 MGI:3773622 MGI Mouse

decreased male germ cell number ( J:89886 )

• mutant testes have fewer proliferating spermatogonia relative to wild-type

• there are increased numbers of apoptotic pachytene and metaphase spermatocytes compared to wild-type

abnormal meiosis ( J:118270 )

• testes show a 3-fold increase in diploid cells, 2-fold increase in tetraploid cells and an 11-fold reduction in haploid cells

abnormal seminiferous tubule morphology ( J:89886 , J:118270 )

• tubules display poor germ cell differentiation, and reduced cellularity, with most germ cell differentiation ceasing at the diplotene stage (J:89886)

• mutant testes show less active cell proliferation per tubule, and fewer proliferative tubules per testis (J:89886)

• no lumen is observed (J:118270)

small testis ( J:118270 )

• testes are normally descended but only attain 23.4% of that in wild-type males

decreased testis weight ( J:89886 )

• size of testes is reduced compared to wild-type males, with weights only one-third of wild-type testes

abnormal spermatogenesis ( J:89886 )

• mice have an increased number of tetraploid cells and a reduction in cells with a haploid DNA content

azoospermia ( J:89886 , J:118270 )

• analysis of epididymes detected only debris instead of normal, motile sperm as observed in wild-type males (J:89886)

• no live sperm are found in epididymis (J:118270)

arrest of male meiosis ( J:89886 , J:118270 )

• diploid to tetraploid germ cell ratio indicates spermatogenic arrest before the first meiosis (J:89886)

• little evidence of meiosis is observed, with no spermatids or spermatozoa, elongated or round detected in the testes (J:89886)

• germ cell development stops at pachytene/diplotene primary spermatocyte stage of the first meiosis division (J:118270)

male infertility ( J:89886 , J:118270 )

• 8-week old males fail to impregnate wild-type females in 3 successive 2-week pairings; vaginal plugs were detected but no pregnancies resulted (J:89886)

• in three successive 2-week pairings with wild-type females, 14-week old males fail to impregnated the females; vaginal plugs were detected in females after mating (J:118270)