Analysis Tools
mortality/aging
| N |
• under normal conditions, homozygotes are viable and fertile, with no apparent defects in growth, external morphology or behavior
• importantly, unwounded skin from homozygotes displays normal tissue architecture and cell organization in the subepithelial, middermal, and deep dermal regions; differences in dermal organization become significant only in the context of the healing process
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immune system
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• peritoneal exudates exhibit decreased numbers of inflammatory cells compared with wild-type exudate
• however, serum white blood cell counts are normal
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• mice fed a high-fat diet exhibit reduced accumulation of macrophages in adipose tissue compared with similarly treated wild-type mice
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• leukocytes from peritoneal exudates of Ang-II-stimulated mice exhibit decreased viability compared with cells from similarly treated wild-type mice
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• leukocytes exhibit decreased basal and absent MCP-1-dependent migration compared with similarly treated wild-type and heterozygous cells
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• microphages are hypomotile with reduced basal motility and absent MCP-1-stimulated motility unlike wild-type cells
• exogenous Spp1 only partially rescues macrophage motility
• migration of macrophages from obese mice towards stromal vascular cells is decreased compared with wild-type cells
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• increased numbers of macrophage in the liver after infection
• peritoneal exudate cells elevated at 72 hours after infection relative to controls
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• compared to in wild-type mice when fed a high-fat diet
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• when fed a high-fat diet, plasma levels of MCP-1 (Ccl2) and PAI-1 (Serpine1) are decreased compared to in similarly treated wild-type mice
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• overall greater burden of granulomas after infection than in controls, 3.1% of liver tissue compared to 1.1%
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• following exposure to Schistosoma mansoni eggs, pulmonary granuloma lesions formation is delayed, granuloma size is reduced at early time points but increased at later time points, and clearance is delayed compared to in similarly treated wild-type mice
• at day 75 of S. mansoni infection, granulomas are composed of mononuclear cells unlike wild-type granulomas with epithelioid morphology
• S. mansoni egg-exposed mice exhibit reduced giant cells in granulomas until day 75 when giant cell formation is increased compared to in similarly treated wild-type mice
• S. mansoni egg-exposed mice exhibit granulomas with few or no macrophages unlike in similarly treated wild-type mice
• however, T cell accumulation in S. mansoni egg granulomas is normal
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• Mycobacterium bovis persists for at least 4 weeks after infections in livers and spleens at levels 10-40X those found in controls
• bacterial loads are reduced by 12 weeks after infection
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homeostasis/metabolism
| N |
• mice exhibit normal serum and urine biochemistry
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• following myocardial infarction, mice exhibit increased left ventricular mid-papillary circumference, lung weight, and left ventricle volume to heart weight ratio with a greater rightward shift in left ventricle pressure-volume relationship and decreases in chamber stiffness constant, myocyte length, and collagen content compared with similarly treated wild-type mice
• however, mice exhibit normal infarct size, heart weight, number of apoptotic myocytes, and survival
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• compared to in wild-type mice when fed a high-fat diet
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• compared to in wild-type mice when fed a high-fat diet
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• compared to in wild-type mice when fed a high-fat diet
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• compared to in wild-type mice when fed a high-fat diet
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• when fed a high-fat diet, mice exhibit a modest increase in respiratory quotient during the 12-hour light cycle compared with similarly treated wild-type mice
• however, mice fed a high-fat diet exhibit a normal respiratory quotient when analyzed over a 24 hour period
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• compared to in wild-type mice when fed a high-fat diet
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• compared to in wild-type mice when fed a high-fat diet
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• when fed a high-fat diet, plasma levels of MCP-1 (Ccl2) and PAI-1 (Serpine1) are decreased compared to in similarly treated wild-type mice
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crystalluria
(
J:103140
)
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• 4 weeks following hyperoxaluria induction with 1% ethylene glycol, mice exhibit CaOx monohydrate crystalluria and retention in renal tubes unlike treated wild-type mice
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• bleomycin-treated mice exhibit an increase in the number and size of cystic epithelial lined air spaces within fibrotic areas of the lung compared to in similarly treated wild-type mice
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• at 2 weeks after dermal incisional wounding, homozygotes exhibit reduced debridement throughout the wound site, esp. in the subepithelial dermis
• in resolving wounds, the reforming matrix appears to be less organized in both the subepithelial and middermal regions
• in the subepithelial and middermal zones, the organization of collagen fibrils and fibers is less distinct
• in the deep reticular dermis, the collagen fibril diameter is significantly smaller; fibrils remain small, with a homogeneous distribution at all wound levels
• notably, homozygotes show no significant differences in tensile strength of healing incisional wounds relative to wild-type mice
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• following carotid artery ligation, mice exhibit a 10-fold decrease leukocyte infiltration, decreased neointimal formation, and greater constrictive remodeling resulting in normal lumen area but decreased medial area compared with similarly treated wild-type mice
• however, cell proliferation and apoptosis during blood vessel healing is normal at 14 days
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cardiovascular system
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• the collagenous matrix around blood vessels is more loosely organized compared to in wild-type mice
• however, elastic fibers surrounding blood vessels are normal
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• mice exhibit reduced Angiotensin II-induced atherosclerotic lesions compared with Apoetm1Unc homozygotes
• bone marrow used to repopulate irradiated Apoetm1Unc homozygotes confers decreased susceptibility to Ang-II-induced atherosclerosis
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• blood flow at similar heart rates is decreased compared to in wild-type mice
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• following myocardial infarction, mice exhibit increased left ventricular mid-papillary circumference, lung weight, and left ventricle volume to heart weight ratio with a greater rightward shift in left ventricle pressure-volume relationship and decreases in chamber stiffness constant, myocyte length, and collagen content compared with similarly treated wild-type mice
• however, mice exhibit normal infarct size, heart weight, number of apoptotic myocytes, and survival
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• vascular tone and compliance are increased compared to in wild-type mice
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• blood vessels dilate rapidly at lower pressures than similarly treated wild-type vessels
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• following carotid artery ligation, mice exhibit a 10-fold decrease leukocyte infiltration, decreased neointimal formation, and greater constrictive remodeling resulting in normal lumen area but decreased medial area compared with similarly treated wild-type mice
• however, cell proliferation and apoptosis during blood vessel healing is normal at 14 days
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renal/urinary system
| N |
• mice exhibit normal urine biochemistry
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crystalluria
(
J:103140
)
|
• 4 weeks following hyperoxaluria induction with 1% ethylene glycol, mice exhibit CaOx monohydrate crystalluria and retention in renal tubes unlike treated wild-type mice
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respiratory system
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• bleomycin-treated mice exhibit an increase in the number and size of cystic epithelial lined air spaces within fibrotic areas of the lung compared to in similarly treated wild-type mice
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• following myocardial infarction
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growth/size/body
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• compared to in wild-type mice when fed a high-fat diet
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• bleomycin-treated mice exhibit an increase in the number and size of cystic epithelial lined air spaces within fibrotic areas of the lung compared to in similarly treated wild-type mice
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• following myocardial infarction
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hematopoietic system
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• peritoneal exudates exhibit decreased numbers of inflammatory cells compared with wild-type exudate
• however, serum white blood cell counts are normal
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• mice fed a high-fat diet exhibit reduced accumulation of macrophages in adipose tissue compared with similarly treated wild-type mice
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• leukocytes from peritoneal exudates of Ang-II-stimulated mice exhibit decreased viability compared with cells from similarly treated wild-type mice
|
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• leukocytes exhibit decreased basal and absent MCP-1-dependent migration compared with similarly treated wild-type and heterozygous cells
|
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• microphages are hypomotile with reduced basal motility and absent MCP-1-stimulated motility unlike wild-type cells
• exogenous Spp1 only partially rescues macrophage motility
• migration of macrophages from obese mice towards stromal vascular cells is decreased compared with wild-type cells
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• increased numbers of macrophage in the liver after infection
• peritoneal exudate cells elevated at 72 hours after infection relative to controls
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muscle
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• blood vessels dilate rapidly at lower pressures than similarly treated wild-type vessels
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cellular
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• leukocytes exhibit decreased basal and absent MCP-1-dependent migration compared with similarly treated wild-type and heterozygous cells
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• increased numbers of macrophage in the liver after infection
• peritoneal exudate cells elevated at 72 hours after infection relative to controls
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