Phenotypes Associated with This Genotype

Genotype
MGI:2676189

• Allelic Composition: Jun^tm1Wag/Jun^tm1Wag
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Jun^tm1Wag/Jun^tm1Wag fetuses exhibit increased apoptosis in the liver

liver/biliary system

increased hepatocyte apoptosis ( J:55508 )

• at E13.0, 8 of 19 mutant fetal livers show significantly increased apoptosis of both hepatoblasts and erythroid cells; however, no deregulation of apoptosis is noted at E12.5

• mutant fetal liver cells are able to reconstitute all hematopoietic compartments of lethally irradiated recipient mice, indicating that hematopoietic cell apoptosis is likely not due to a cell-autonomous defect

• in vitro, primary hepatoblasts and fibroblasts obtained from E12.5 homozygotes show a ~4-fold increase in apoptosis relative to wild-type cells

decreased hepatocyte proliferation ( J:55508 )

• in vitro, primary hepatoblasts and fibroblasts obtained from E12.5 homozygotes display a ~50% reduction in mitotic capacity as shown by [³H]thymidine incorporation

cardiovascular system

right aortic arch ( J:55508 )

• at E12.5, some homozygotes display abnormal remodeling of the aortic arch arteries resulting in a right-sided aortic arch

persistent truncus arteriosus ( J:55508 )

• at E12.5, all (19 of 19) homozygotes exhibit a single outflow vessel arising from the right ventricle

ventricular septal defect ( J:55508 )

• at E14.5, homozygotes display incomplete septation of the left and right ventricles

decreased heart right ventricle wall thickness ( J:55508 )

• at E12.5, homozygotes display a thinner right ventricular wall

embryo

abnormal cardiac neural crest cell migration ( J:55508 )

• at E12.5, homozygotes exhibit reduced migration of neural crest cells in the outflow tract of the right ventricle

cellular

increased hepatocyte apoptosis ( J:55508 )

• at E13.0, 8 of 19 mutant fetal livers show significantly increased apoptosis of both hepatoblasts and erythroid cells; however, no deregulation of apoptosis is noted at E12.5

• mutant fetal liver cells are able to reconstitute all hematopoietic compartments of lethally irradiated recipient mice, indicating that hematopoietic cell apoptosis is likely not due to a cell-autonomous defect

• in vitro, primary hepatoblasts and fibroblasts obtained from E12.5 homozygotes show a ~4-fold increase in apoptosis relative to wild-type cells

abnormal cardiac neural crest cell migration ( J:55508 )

• at E12.5, homozygotes exhibit reduced migration of neural crest cells in the outflow tract of the right ventricle

decreased hepatocyte proliferation ( J:55508 )

• in vitro, primary hepatoblasts and fibroblasts obtained from E12.5 homozygotes display a ~50% reduction in mitotic capacity as shown by [³H]thymidine incorporation