Jun^tm1Wag
Targeted Allele Detail

Summary

• Symbol: Jun^tm1Wag
• Name: jun proto-oncogene; targeted mutation 1, Erwin F Wagner
• MGI ID: MGI:2149630
• Synonyms: c-jun-
• Gene: Jun Location: Chr4:94937273-94940459 bp, - strand Genetic Position: Chr4, 43.34 cM, cytoband C5-C7
• Alliance: Jun^tm1Wag page

Jun^tm1Wag/Jun^tm1Wag fetuses exhibit increased apoptosis in the liver

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:14538
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 703 bp of sequence that codes for the DNA binding and leucine zipper domains. Western blot analysis of E9.5 lysates did not detect wild-type protein in homozygous mutant embryos. (J:14538)

Expression

In Mice Carrying this Mutation:	40 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jun Mutation: 12 strains or lines available

Notes

ES cell line = D3-2. Mutant ES cells failed to contribute to the liver but not to other tissues of adult chimeric mice (J:14538). A developmental analysis of chimeras showed contribution of mutant ES cell derivatives to fetal, but not to adult livers, suggesting a role in hepatocyte turnover (J:55508).

References

• Original: J:14538 Hilberg F, et al., c-jun is essential for normal mouse development and hepatogenesis [published erratum appears in Nature 1993 Nov 25;366(6453):368]. Nature. 1993 Sep 9;365(6442):179-81
• All: 6 reference(s)